Product Name:
PTPN21-pS637
Product Number:
ab-pp516
Target Full Name: Tyrosine-protein phosphatase non-receptor type 21
Target Alias: EC 3.1.3.48; Protein tyrosine phosphatase, non-receptor type 21; Protein-tyrosine phosphatase D1; PTN21; PTPD1; PTPRL10; Tyrosine-protein phosphatase non-receptor type 21
Product Type Specific: PTPN21 protein phosphatase phosphosite-specific antibody
Antibody Code: PP516
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S637
Protein UniProt: Q16825
Protein SigNET: PTPN21
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPN21
Antibody Immunogen Sequence: KRN(pS)IEVA(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues K634 to A641. The effect of S637 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PTPN21 (≥27 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is conserved in mammals and also found in chicken.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken.
Related Product 1: PTPN22 S637 phosphorylation antibody (Cat. No.: AB-PP517)
Related Product 2: PTPN23 Y499 phosphorylation antibody (Cat. No.: AB-PP518)
Related Product 3: PTPN23 Y1229 phosphorylation antibody (Cat. No.: AB-PP537)
Scientific Background: PTPN21 (Protein Tyrosine Phosphatase Non-Receptor Type 21; PTPD1) belongs to the protein-tyrosine phosphatase family, Non-receptor class subfamily. It regulates critical cellular processes including growth, differentiation, cell adhesion, spreading, and migration. It features an N-terminal FERM domain that facilitates cytoskeletal-plasma membrane interactions and a C-terminal phosphatase domain. PTPN21 interacts with proteins like BMX/ETK (increasing STAT3 activation) and KIF1C, which regulates trafficking. It plays a role in maintaining hematopoietic stem cell (HSC) homeostasis and retention in the niche by regulating cellular mechanics through the dephosphorylation of Septin1. It is found in both the cytoplasm and associated with the cytoskeleton. PTPN21 is often up-regulated in various cancers, and has been implicated in promoting cancer cell invasion. This description may include information annotated by UniProt and/or Google AI.