Product Name:
PTPN2-pS304
Product Number:
ab-pp515
Target Full Name: Tyrosine-protein phosphatase non-receptor type 2; T-cell protein-tyrosine phosphatase
Target Alias: EC 3.1.3.48; Protein tyrosine phosphatase, non-receptor type 2; PTN2; PTPT; T-cell protein-tyrosine phosphatase; TCELLPTP; TCPTP; TC-PTP
Product Type Specific: PTPN2 protein phosphatase phosphosite-specific antibody
Antibody Code: PP515
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S304
Protein UniProt: P17706
Protein SigNET: PTPN2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPN2
Antibody Immunogen Sequence: FDH(pS)PNK(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F301 to K307. The effect of S304 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PTPN2 (≥40 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in fish. PTPN2 is known to be phosphorylated at this site in vitro by CDK1 (CDC2), and CDK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse.
Related Product 1: PTP1B Y20 phosphorylation antibody (Cat. No.: AB-PP532)
Related Product 2: PTP1B Y46 phosphorylation antibody (Cat. No.: AB-PP533)
Related Product 3: PTP1B S50 phosphorylation antibody (Cat. No.: AB-PP510)
Related Product 4: PTP1B Y362 phosphorylation antibody (Cat. No.: AB-PP509)
Related Product 5: PTPN3 Y1165 phosphorylation antibody (Cat. No.: AB-PP519)
Scientific Background: PTPN2 (Protein Tyrosine Phosphatase Non-Receptor Type 2; TC-PTP) is a cytosolic/nuclear enzyme that belongs to the protein-tyrosine phosphatase family, Non-receptor class 1 subfamily. It functions as a key negative regulator of immune, inflammatory, and cancer signaling pathways. It dephosphorylates tyrosine kinases (e.g., INSR, EGFR, CSF1R, PDGFR, JAK1, JAK2, JAK3, Src family kinases) to control cell growth, survival, and differentiation, while acting as a crucial tumour suppressor. It is a major regulator of cytokine signaling, specifically the JAK-STAT pathway, by dephosphorylating STAT1, STAT3, and STAT5a/b. It also inactivates growth factor receptors like EGFR. PTPN2 regulates the development and function of immune cells (T-cells, B-cells, macrophages). Deficiencies are associated with autoimmune diseases, including type 1 diabetes. It features a single catalytic domain and a nuclear localization sequence (NLS), allowing it to function in both the cytoplasm and nucleus. It is ubiquitously expressed with Isoform 2 as the most common, and Isoform 1 is expressed in T-cells and in placenta. PTPN2 functions as a tumour suppressor in T-cell acute lymphoblastic leukemia (T-ALL). Loss-of-function in tumour cells enhances the immune system's ability to fight cancer, particularly when combined with immunotherapy. In addition, PTPN2 dysfunction is linked to chronic inflammation, including Crohn's disease and rheumatoid arthritis. This description may include information annotated by UniProt and/or Google AI.