Product Name:
PPPM1A-pY362
Product Number:
ab-pp508
Target Full Name: Protein phosphatase 1A; Protein phosphatase 2C isoform alpha
Target Alias: EC 3.1.3.16; IA; P2CA; PP2CA; PP2C-alpha; PPM1A
Product Type Specific: PPPM1A protein phosphatase phosphosite-specific antibody
Antibody Code: PP508
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y362
Protein UniProt: P35813
Protein SigNET: PPPM1A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPPM1A
Antibody Immunogen Sequence: EAV(pY)NRL(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E359 to L365. The effect of Y362 phosphorylation is unclear. This is one of the major sites of in vivo phosphorylation of PP2Ca based on ≥9 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: PPM1B Y367 phosphorylation antibody (Cat. No.: AB-PP540)
Related Product 2: PPP1CA Y134+Y137 phosphorylation antibody (Cat. No.: AB-PP541)
Related Product 3: PPP1CA Y306 phosphorylation antibody (Cat. No.: AB-PP542)
Related Product 4: PPP1CB T66 phosphorylation antibody (Cat. No.: AB-PP502)
Related Product 5: PPP1CB Y306 phosphorylation antibody (Cat. No.: AB-PP530)
Related Product 6: PPP2CA Y284 phosphorylation antibody (Cat. No.: AB-PP549)
Related Product 7: PPP2CA T646 phosphorylation antibody (Cat. No.: AB-PP504)
Related Product 8: PPP2CB T304 phosphorylation antibody (Cat. No.: AB-PP505)
Scientific Background: PPM1A (Protein phosphatase, Mg2+/Mn2+ Dependent 1A), also known as PP2Cα, is a nuclear enzyme that acts as a serine/threonine protein phosphatase. As a member of the PP2C family, it generally functions as a negative regulator in various signaling pathways, opposing the role of protein kinases. PPM1A consists of an N-terminal catalytic domain (common to all PPMs) and a 90-residue C-terminal domain. It is a monomeric, metal-dependent enzyme that requires Mg2+ or Mn2+ for activity. PPM1A negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. It directly dephosphorylates YAP/TAZ (key effectors of Hippo signaling), facilitating their nuclear localization and transcriptional activity, which is essential for liver and intestinal regeneration. It inhibits NF-κB by directly dephosphorylating the p65 subunit (RelA) at Ser-536 or Ser-276 and inhibiting upstream IKKs. It acts as a negative regulator of stress-activated protein kinases (MAPKs) by dephosphorylating JNK and p38, as well as their upstream activators MKK4/7 and MKK3/6. It is involved in dephosphorylating CDKs (such as CDK2 and CDK6) and acts on AMPK (AMP-activated protein kinase). It is expressed in almost all tissues and is found in both the cytoplasm and the nucleus. PPM1A is frequently considered a tumour suppressor. Its downregulation is associated with increased invasiveness, epithelial-mesenchymal transition (EMT), and poor prognosis in various cancers, including bladder, breast (ER-negative), and hepatocellular carcinoma. Due to its role in inhibiting TGF-β1/Smad3 signaling, PPM1A acts as an anti-fibrotic molecule in the kidney and liver. It is known to regulate innate immune homeostasis by dephosphorylating STING and TBK1, and its upregulation can impair antibacterial responses during infections like Mycobacterium tuberculosis. It is linked to the pathogenesis of Ankylosing Spondylitis (AS), where it acts as a damage-associated molecular pattern (DAMP) to promote inflammation. This description may include information annotated by UniProt and/or Google AI.