Product Name:
PPP5C-pY119
Product Number:
ab-pp507
Target Full Name: Serine/threonine-protein phosphatase 5
Target Alias: EC 3.1.3.16; PP5; PPP5; PPT; PP-T; Protein phosphatase 5, catalytic subunit; Protein phosphatase T; Serine/threonine protein phosphatase 5
Product Type Specific: PPP5C protein phosphatase phosphosite-specific antibody
Antibody Code: PP507
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y119
Protein UniProt: P53041
Protein SigNET: PPP5C
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP5C
Antibody Immunogen Sequence: LRD(pY)ETV(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues L116 to V122. The effect of Y119 phosphorylation is unclear. This is one of the minor sites of in vivo phosphorylation of PPP5C (≥12 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This particular human phosphosite is highly conserved in vertebrates and also found in the sea urchin.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Honey bee | Sea urchin.
Related Product 1: PP5 (PPT) expression antibody (Cat. No.: AB-NP021-3)
Related Product 2: PP5 (PPT) expression antibody (Cat. No.: AB-NP021-4)
Scientific Background: PPP5C (Protein Phosphatase 5 Catalytic Subunit) is a conserved serine/threonine phosphatase that belongs to the PPP phosphatase family, PP-5 (PP-T) subfamily. It is characterized by an N-terminal tetratricopeptide repeat (TPR) domain that regulates its activity. It is implicated in the regulation of a wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. It acts as a key molecular switch in signaling pathways—including stress response and cell growth—by dephosphorylating targets like CSNK1E, ASK1/MAP3K5, PRKDC, RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT, DNA-PKcs, and tumour suppressors. It functions as both a phosphatase and a co-chaperone for Heat Shock Protein 90 (Hsp90), modulating protein folding and signaling. It participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. It inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. It plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. It dephosphorylates and inhibits the anti-adipogenic effect of NR3C1. It regulates the circadian rhythms, through the dephosphorylation and activation of CSNK1E. It modulates TGF-beta signaling pathway by the regulation of SMAD3 phosphorylation and protein expression levels. It dephosphorylates TAU/MAPT. Through their dephosphorylation, PPP5C may play a role in the regulation of ions channels such as KCNH2. Its catalytic activity is usually autoinhibited by the N-terminal TPR domain, which relaxes upon binding to Hsp90 or fatty acids. PPP5C is ubiquitously expressed throughout mammalian tissues, with particularly high levels found in the brain. Elevated levels are linked to breast and kidney cancers, while dysfunction is implicated in neurodevelopmental disorders, such as epilepsy, and Alzheimer's disease. This description may include information annotated by UniProt and/or Google AI.