Product Name:
PPP3CC-pS463
Product Number:
ab-pp506
Target Full Name: Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform
Target Alias: Calcineurin A gamma; Calcineurin, testis-specific catalytic subunit; CALNA3; CAM- PRP catalytic subunit; EC 3.1.3.16; P2BC; PP2BC; PP2B-gamma
Product Type Specific: PPP3CC protein phosphatase phosphosite-specific antibody
Antibody Code: PP506
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S463
Protein UniProt: P48454
Protein SigNET: PPP3CC
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP3CC
Antibody Immunogen Sequence: KIR(pS)FEE(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues K460 to E466. The effect of S463 phosphorylation is unclear. This is one of the minor sites of in vivo phosphorylation of PPP3CC (≥7 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This particular human phosphosite is highly conserved in vertebrates and also found in the fruit fly.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Frog | Zebra fish | Fruit fly.
Related Product 1: PP5 (PPT) expression antibody (Cat. No.: AB-NP021-3)
Related Product 2: PP5 (PPT) expression antibody (Cat. No.: AB-NP021-4)
Related Product 3: PPP5C S463 phosphorylation antibody (Cat. No.: AB-PP507)
Related Product 4: PPP6C Y261 phosphorylation antibody (Cat. No.: AB-PP553)
Scientific Background: PPP3CC (Protein Phosphatase 3 Catalytic Subunit Gamma) is the gamma isoform of the catalytic subunit of calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase, and belongs to the PPP phosphatase family, PP-2B subfamily. It acts in a complex with regulatory subunits (calcineurin B) and calmodulin, which bind Ca2+. It plays an important role in regulating intracellular Ca2+-mediated signals, including synaptic vesicle cycling and dopaminergic signaling, synaptic plasticity, and regulating sperm motility. It dephosphorylates key targets like the transcription factors NFATC1 and ELK1, which are inactivated, as well as DARPP32. PPP3CC is notably expressed in the testis and is also found in the brain, with, for example, high expression in the presynaptic terminals of hippocampal neurons. Variations in PPP3CC are linked to schizophrenia, indicating a role in the progression of psychiatric disorders through altered signaling. This description may include information annotated by UniProt and/or Google AI.