Product Name:
PPP2CB-pT304
Product Number:
ab-pp505
Target Full Name: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
Target Alias: EC 3.1.3.16; P2AB; PP2AB; PP2A-beta; Protein phosphatase 2 (formerly 2A) catalytic subunit, beta isoform; Serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
Product Type Specific: PPP2CB protein phosphatase phosphosite-specific antibody
Antibody Code: PP505
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T304
Protein UniProt: P62714
Protein SigNET: PPP2CB
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP2CB
Antibody Immunogen Sequence: TRR(pT)PDY(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues T301 to Y307. T304 phosphorylation inhibits phosphatase activity. This is one of the major sites of in vivo phosphorylation of PPP2CB based on ≥10 mass spectrometry reports recorded in PhosphoSitePlus. This particular human phosphosite is highly conserved in vertebrates and also found in yeast and mould.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Fruit fly | Honey bee | Sea urchin | Thale cress | Budding yeast | Red bread mold.
Related Product 1: PPP2CA T646 phosphorylation antibody (Cat. No.: AB-PP504)
Related Product 2: PPP2CA Y284 phosphorylation antibody (Cat. No.: AB-PP549)
Scientific Background: PPP2CB (Protein Phosphatase 2 Catalytic Subunit Beta) belongs to the PPP phosphatase family, PP-1 subfamily. It is a major serine/threonine phosphatase that acts as a tumour suppressor by regulating cell growth, division, and signaling pathways. It functions as the catalytic subunit (C-beta) of Protein Phosphatase 2A (PP2A), dephosphorylating targets to control processes like apoptosis and microtubule dynamics. It is predominantly found in the cytoplasm and is part of the heteromeric PP2A enzyme, which binds to regulatory subunits to manage substrate specificity. As a core subunit of PP2A, PPP2CB modulates through dephosphorylation, the activity of kinases (e.g., Akt, MAP-2 kinase, phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase) and regulates crucial signaling, such as the Hippo and TGF-beta pathways. PPP2CB is crucial for maintaining cellular homeostasis, and its dysfunction is associated with malignant cancer transformation and neurodegenerative diseases such as Alzheimer's disease. This description may include information annotated by UniProt and/or Google AI.