Product Name:
HePTP-pS44
Product Number:
ab-pp500
Target Full Name: Tyrosine-protein phosphatase non-receptor type 7
Target Alias: EC 3.1.3.48; LC-PTP; Protein-tyrosine phosphatase, non-receptor type 7; PTN7; PTPN7
Product Type Specific: HePTP protein phosphatase phosphosite-specific antibody
Antibody Code: PP500
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S44
Protein UniProt: P35236
Protein SigNET: HePTP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HePTP
Antibody Immunogen Sequence: RRG(pS)NVA(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R41 to A47. S44 phosphorylation inhibits interaction with ERK2. This is one of the major in vivo phosphorylation sites in HePTP (≥44 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and found in fish. HePTP is known to be phosphorylated at this site in vitro by PKACa (PRKACA), and PKCt (PRKCQ, PKCq).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish .
Related Product 1: HePTP S143 phosphorylation antibody (Cat. No.: AB-PP528)
Scientific Background: HePTP (haematopoietic protein tyrosine phosphatase; tyrosine-protein phosphatase non-receptor type 7; PTPN7) belongs to a subgroup of phosphatases that include STEP and PTP-SL, which regulate MAPKs in different tissues. It is a 38 kDa non-receptor protein-tyrosine phosphatase that is primarily expressed in T-cells, B-cells, and hematopoietic tissues such as thymus and spleen. It consists of a C-terminal catalytic domain (residues 44-339) and an N-terminal extension (residues 1-43), which features a Kinase Interaction Motif (KIM). The KIM enables high-affinity binding to ERK1/2 and p38 MAP kinases, and allows HePTP to dephosphorylate the activating phospho-Tyr-185 in their activation loops. HePTP activity is regulated by PKA-mediated phosphorylation at Ser-23, which causes the phosphatase to dissociate from ERK2/p38, allowing the MAPKs to translocate to the nucleus. It is highly expressed in acute myeloid leukemia. This description may include information annotated by UniProt and/or Google AI.