Product Name:
SYT1-pY312
Product Number:
ab-pn998
Target Full Name: Synaptotagmin-1
Target Alias: P65; SVP65; SYT
Product Type Specific: SYT1 phosphosite-specific antibody
Antibody Code: PN998
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y312
Protein UniProt: P21579
Protein SigNET: SYT1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SYT1
Antibody Immunogen Sequence: C(βA)SDP(pY)VKIH
Antibody Immunogen Description: Corresponds to amino acid residues S309 to H316. The effect of SYT1 Y312 phosphorylation is unclear. This is a major phosphorylation site in SYT1 based on ≥43 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish | Fruit fly | Honey bee | Nematode worm.
Related Product 1: SYT1 T129 phosphorylation antibody (Cat. No.: AB-PN997)
Related Product 2: SYT1 Y381 phosphorylation antibody (Cat. No.: AB-PN999)
Scientific Background: SYT1 (Synaptotagmin-1) is an essential Ca2+-sensing, integral membrane protein of synaptic vesicles that belongs to the synaptotagmin family. Upon Ca2+ influx, it mediates fast exocytosis (neurotransmitter release) through regulation of membrane interactions for trafficking of synaptic vesicles at the active zone of the synapse. It triggers synchronized neurotransmitter release by mediating Ca2+-dependent vesicle docking and fusion with the SNARE complex. It features a luminal domain, a transmembrane domain, and two cytosolic C2 domains (C2A and C2B). The C2A and C2B domains bind Ca2+ and anionic phospholipids, which require the presence of both an acidic head group and a diacylglycerol backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C (PKC) also exists. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes, where it is expressed. Mutations in the SYT1 gene are linked to a severe neurodevelopmental syndrome, characterized by developmental delay, involuntary movements, and hypotonia. This description may include information annotated by UniProt and/or Google AI.