Product Name:
SYNJ1-pS1084
Product Number:
ab-pn995
Target Full Name: Synaptojanin-1; Synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1
Target Alias: INPP5G; Synaptic inositol-1,4,5-trisphosphate 5- phosphatase 1; Synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1; Synaptic inositol-1;4;5-trisphosphate 5-phosphatase 1; Synaptojanin 1
Product Type Specific: SYNJ1 phosphosite-specific antibody
Antibody Code: PN995
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1084
Protein UniProt: O43426
Protein SigNET: SYNJ1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SYNJ1
Antibody Immunogen Sequence: SAQS(pS)PIDAC
Antibody Immunogen Description: Corresponds to amino acid residues S1080 to A1088. The effect of SYNJ1 S1084 phosphorylation is unclear. This is a major site of in vivo phosphorylation of SYNJ1 based on ≥14 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.65 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Related Product 1: SYNJ1 Y784+Y786 phosphorylation antibody (Cat. No.: AB-PN996)
Scientific Background: SYNJ1 (Synaptojanin) is a critical phosphoinositide phosphatase that is highly enriched in the brain. It catalyzes the breaks down PI(4,5)P2 to PI(4)P or phosphatidylinositol. By regulating membrane PI(4,5)P2 levels, it is essential for synaptic vesicle endocytosis (recycling) and endocytosis and clathrin-mediated endocytosis, allowing vesicles to be recycled for further neurotransmitter release. It also plays a role in autophagosome biogenesis. It is composed of three main domains: an N-terminal Sac1-like inositol domain, a central 5′-phosphatase domain (5′PP), and a C-terminal region that binds to endocytic proteins like clathrin. The two phosphatase domains (Sac1-like and 5-phosphatase) dephosphorylate lipids. SYNJ1 is located in the presynaptic terminal, attached to synaptic vesicles and endocytic intermediates. Loss of function mutations of SYNJ1 is linked to early-onset Parkinson's disease (e.g. PARK20), and early-onset epilepsy, It has also been implicated in Alzheimer's disease, where it may become insoluble, and in Down syndrome due to gene overexpression. This description may include information annotated by UniProt and/or Google AI.