Product Name:
STXBP1-pT574
Product Number:
ab-pn993
Target Full Name: Syntaxin-binding protein 1
Target Alias: HUNC18; Munc18-1; N- Sec1; N-Sec1; P67; RbSec1; STB1; STXB1; Syntaxin binding protein 1; UNC18; Unc-18 homolog; Unc-18-1; UNC18A; Unc-18A
Product Type Specific: STXBP1 phosphosite-specific antibody
Antibody Code: PN993
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T574
Protein UniProt: P61764
Protein SigNET: STXBP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STXBP1
Antibody Immunogen Sequence: CSTHIL(pT)PQK
Antibody Immunogen Description: Corresponds to amino acid residues S569 to K577. Phosphorylation of T574 inhibits exocytosis. This has not been identified as an in vivo phosphorylation site of STX1B based mass spectrometry reports recorded in PhosphoSitePlus. STXBP1 is known to be phosphorylated at this site in vitro by CDK5, ERK1 (MAPK3), and ERK2 (MAPK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken | Frog | Fruit fly | Honey bee | Nematode worm | Sea urchin .
Related Product 1: STXBP1 S313 phosphorylation antibody (Cat. No.: AB-PN992)
Related Product 2: STXBP1 Y473 phosphorylation antibody (Cat. No.: AB-PN994)
Scientific Background: STXBP1 (Syntaxin-binding protein 1; Munch18-1) is a regulatory chaperone protein that belongs to the STXBP/unc-18/SEC1 family. It participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery in the SNARE complex probably in a 1:1 ratio. It can interact with syntaxins 1, 2, and 3, but not with syntaxin 4. It is an arch-shaped, 68-kDa protein that forms a cavity to bind the Habc domain and the SNARE motif of syntaxin-1. It may serve a role in determining the specificity of intracellular fusion reactions. It is highly expressed in brain and spinal cord, and particularly enriched in axons. Pathogenic mutations causing haploinsufficiency—where not enough functional protein is produced—lead to intellectual disability, developmental delays, and STXBP1-related disorders such as STXBP1-RD), which include infantile epileptic encephalopathy-4 (also known as Ohtahara syndrome). Symptoms of deficiency of STXBP1 include seizures (85-90% of patients), severe intellectual disability, motor impairment (ataxia, tremor, hypotonia), and autism spectrum disorder features. STXBP1 disruption can also leads to an imbalance in neurotransmission, creating excessive, uncontrolled neuron excitation. This description may include information annotated by UniProt and/or Google AI.