Product Name:
SNAP25-pT138
Product Number:
ab-pn991
Target Full Name: Synaptosomal-associated protein 25
Target Alias: Bdr; RIC4; SNAP25; SNAP-25B; SNP25; SUP; super protein; synaptosomal associated 25kDa protein
Product Type Specific: SNAP25 synaptic phosphosite-specific antibody
Antibody Code: PN991
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T138
Protein UniProt: P60880
Protein SigNET: SNAP25
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SNAP25
Antibody Immunogen Sequence: IRRV(pT)NDA(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues I134 to A1413. The effect of SNAP25 T138 phosphorylation is unclear. This is the major in vivo site for phosphorylation of SNAP25 based on ≥8 mass spectrometry reports recorded in PhosphoSitePlus. SNAP25 is known to be phosphorylated at this site in vitro by PKACa (PRKACA), PKCa (PRKCA), and ROCK2 (ROK-alpha).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Honey bee | Sea urchin.
Related Product 1: SNAP25 expression antibody (Cat. No.: AB-NN327-1)
Related Product 2: SNAP25 S28+T29 phosphorylation antibody (Cat. No.: AB-PN1003)
Related Product 3: SNAP25 S187 phosphorylation antibody (Cat. No.: AB-PN990)
Scientific Background: SNAP25 (Synaptosomal-associated protein 25) is a 25 kDa membrane bound protein anchored to the cytosolic face of membrane via palmitoyl side chains in the middle of the molecule (1). It functions in the regulation of neurotransmitter release, acting as a key component of the SNARE complex to facilitate synaptic vesicle fusion at the presynaptic plasma membrane. It is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together (2). It forms a core four alpha-helix bundle with syntaxin-1 and synaptobrevin (VAMP) to drive the docking and fusion of vesicles with the membrane. SNAP25 exists as two alternatively spliced isoforms, SMAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein (3). SNAP25 is anchored to the cytoplasmic face of the plasma membrane via palmitoylated cysteine residues, rather than a transmembrane domain. Mutations or imbalances in SNAP25 are associated with epilepsy, intellectual disability (SNAP25-DEE), and neuropsychiatric disorders like ADHD. Botulinum Toxin Type A (BoNT-A) specifically cleaves SNAP-25, blocking acetylcholine release and inhibiting muscle contraction. This description may include information annotated by UniProt and/or Google AI.