Product Name:
Shc4-pY424
Product Number:
ab-pn988
Target Full Name: SHC (Srcy 2 domain containing) family, member 4
Target Alias: rai-like protein; RaLP; SHC (Src homology 2 domain containing) family, member 4; SHC (Srcy 2 domain containing) family, member 4
Product Type Specific: Shc4 phosphosite-specific antibody
Antibody Code: PN988
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y424
Protein UniProt: Q6S5L8
Protein SigNET: Shc4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Shc4
Antibody Immunogen Sequence: CSSV(pY)ENA
Antibody Immunogen Description: Corresponds to amino acid residues C420 to A426. Phosphorylation of Y424 induces interaction with Grb2 and SHP2 PTPN11). This is the major in vivo site for phosphorylation of Shc4 based on ≥44 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Mouse.
Related Product 1: Shc1 Y349+Y350 phosphorylation antibody (Cat. No.: AB-PN985)
Related Product 2: Shc1 Y427 phosphorylation antibody (Cat. No.: AB-PN986)
Related Product 3: Shc3 Y406 phosphorylation antibody (Cat. No.: AB-PN987)
Scientific Background: Shc4 (SHC Adaptor Protein 4; ShcD; RaLP) is a 630-amino acid protein belonging to the Shc family of phospho-tyrosine-binding adapter proteins. It is primarily expressed in the brain, spinal cord, and skeletal muscle, regulating receptor tyrosine kinase (RTK) signaling, neuromuscular junction (NMJ) clustering of acetylcholine receptors, and tumour cell migration/invasion. It functions in anchoring acetylcholine receptors at postsynaptic NMJs, specifically interacting with MuSK (muscle-specific kinase). It plays a role as a modulator of the epidermal growth factor receptor (EGFR) signaling cascade, promoting ligand-independent hyperphosphorylation and regulating receptor trafficking. It contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1. It is found in axons and somata of mature neurons, particularly in the hippocampus, cerebellum, and olfactory bulb. It is also expressed in melanomas and other tumours, where it activates Ras-dependent and -independent migration pathways, contributing to cell proliferation and metastasis. It is highly expressed at the transition from radial growth phase to vertical growth phase and metastatic melanomas, when tumour cells acquire migratory competence and invasive potential. This description may include information annotated by UniProt and/or Google AI.