Product Name:
Shc3-pY406
Product Number:
ab-pn987
Target Full Name: SHC (Srcy 2 domain containing) transforming protein 3
Target Alias: Neuronal Shc; NSHC; N-Shc; Protein Rai; SH2 domain protein C3; SHC (Srcy 2 domain containing) transforming protein 3; SHC transforming protein 3; SHCC; Src homology 2 domain containing transforming protein C3
Product Type Specific: Shc3 phosphosite-specific antibody
Antibody Code: PN987
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y406
Protein UniProt: Q92529
Protein SigNET: Shc3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Shc3
Antibody Immunogen Sequence: CSSDI(pY)STP
Antibody Immunogen Description: Corresponds to amino acid residues S402 to P409. The effect of Shc3 Y406 phosphorylation is unclear. This is the major in vivo site for phosphorylation of Shc3 based on ≥28 mass spectrometry reports recorded in PhosphoSitePlus. Shc3 is known to be phosphorylated at this site in vitro by EGFR, Src, TrkA (NTRK1), and TrkB (NTRK2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.55 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat.
Related Product 1: Shc1 Y349+Y350 phosphorylation antibody (Cat. No.: AB-PN985)
Related Product 2: Shc1 Y427 phosphorylation antibody (Cat. No.: AB-PN986)
Related Product 3: Shc4 Y424 phosphorylation antibody (Cat. No.: AB-PN988)
Scientific Background: Shc3 (Src homology and collagen homolog 3; Rai; N-Shc) is a member of the Shc adaptor protein family that acts as a signaling hub for cell survival, differentiation, and migration. The gene produces two main isoforms, p52Shc3 and p64Shc3. It is primarily expressed in neurons, and it is important in neuronal stress responses and neurotrophin-activated pathways. Shc3 is essential for neuron survival in the central nervous system (CNS) and regulates NMDA receptor function. It is involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons. It helps cells adapt to low-oxygen environments with the hypoxia response. It features phosphotyrosine-binding (PTB) and SH2 domains, allowing it to bind to activated growth factor receptors (like Trk or ErbB2) and recruit downstream signaling molecules like PI3K/Akt or MEK/ERK. It is predominantly found in brain and barely detectable in other tissues, except in pancreas. It is highly expressed in the cerebral cortex, frontal and temporal lobes, occipital pole, hippocampus, caudate nucleus and amygdala.It is expressed at low level in the cerebellum, medulla and spinal cord. Shc3 is often overexpressed in various malignancies (e.g., glioblastoma, hepatocellular carcinoma), contributing to tumour progression, angiogenesis, and drug resistance. It promotes tumour stemness, migration, and resistance to therapies in cancers like hepatocellular carcinoma (HCC) and neuroblastoma. . This description may include information annotated by UniProt and/or Google AI.