Product Name:
Shc1-pY427
Product Number:
ab-pn986
Target Full Name: SHC (Src homology 2 domain containing) transforming protein 1
Target Alias: SHC (Src homology 2 domain containing) transforming protein 1; Src homology 2 domain-containing-transforming protein C1
Product Type Specific: Shc1 phosphosite-specific antibody
Antibody Code: PN986
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y427
Protein UniProt: P29353
Protein SigNET: Shc1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Shc1
Antibody Immunogen Sequence: DDPS(pY)VNV(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues D423 to V430. Shc1 Y427 phosphorylation inhibits interaction with PPP2CA, and regulates cell growth, apoptosis, and cell motility. This is the major in vivo site for phosphorylation of Shc1 based on ≥2058 mass spectrometry reports recorded in PhosphoSitePlus. Shc1 is known to be phosphorylated at this site in vitro by EGFR, FAK (PTK2), Lck, Met (HGFR), PDGFRA, Src, and ZAP70.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Zebra fish.
Related Product 1: Shc1 Y349+Y350 phosphorylation antibody (Cat. No.: AB-PN985)
Related Product 2: Shc3 Y406 phosphorylation antibody (Cat. No.: AB-PN987)
Related Product 3: Shc4 Y424 phosphorylation antibody (Cat. No.: AB-PN988)
Scientific Background: Shc1 (Shc-transforming protein 1) is a key cytoplasmic signaling adapter protein that links activated receptor tyrosine kinases (RTKs) to intracellular pathways, including Ras/MAPK, regulating cell growth, differentiation, migration, and apoptosis. It produces three main isoforms (p46Shc, p52Shc, p66Shc) involved in either mitogenic signaling or oxidative stress responses. Shc1 acts as a bridge, utilizing its PTB (phosphotyrosine-binding) and SH2 (Src homology 2) domains to bind activated receptors and initiate downstream signaling, particularly the GRB2/SOS complex activation. p46Shc and p52Shc are primarily involved in transmitting mitotic signals from receptors to the Ras pathway. p46Shc is specifically targeted to the mitochondrial matrix. p66Shc regulates cellular responses to oxidative stress, apoptosis, and lifespan. It acts as a producer of reactive oxygen species (ROS) within mitochondria. Isoform p66Shc acts as a downstream target of the tumour suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span. It participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Shc1 is widely expressed, with high expression in neural stem cells but absence in mature neurons. It is crucial for transmitting signals for growth factors, such as EGF. Shc1 is implicated in cancer (breast cancer, multiple endocrine neoplasia) and cardiovascular diseases, playing a significant role in tumour growth, angiogenesis, and cell senescence. This description may include information annotated by UniProt and/or Google AI.