Product Name:
SAC3-pY631
Product Number:
ab-pn984
Target Full Name: Polyphosphoinositide phosphatase
Target Alias: Fig4; EC 3.1.3.-; Phosphatidylinositol 3,5-bisphosphate 5-phosphatase; SAC domain-containing protein 3
Product Type Specific: SAC3 (FIG4) phosphorylated inositol phosphatase phosphosite-specific antibody
Antibody Code: PN984
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y631
Protein UniProt: Q92562
Protein SigNET: SAC3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SAC3
Antibody Immunogen Sequence: C(βA)RRS(pY)TYWW
Antibody Immunogen Description: Corresponds to amino acid residues R628 to W635. The effect of Y631 phosphorylation is unclear. This is the major site of in vivo phosphorylation of SAC3 based on ≥27 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Mouse | Chicken.
Scientific Background: Fig4/Sac3 phosphatase, a 97-kDa protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles and lysosomal function. As part of a complex with PIKfyve and VAC14, it maintains endolysosomal membrane homeostasis. The protein features a SAC-like domain (approx. 400 amino acids) that is essential for its phosphoinositide phosphatase activity, which is conserved across eukaryotes. Besides its lipid activity, FIG4 has serine-protein phosphatase activity that stimulates PIKfyve, contributing to the synthesis of PI(3,5)P2. It is highly expressed in brain and broadly found in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signalling. Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy. Mutations of Fig4 have been linked with Charcot-Marie-Tooth Disease Type 4J (CMT4J), Yunis-Varon Syndrome, Amyotrophic Lateral Sclerosis and Primary Lateral Sclerosis, and Leukoencephalopathy. This description may include information annotated by UniProt and/or Google AI.