Product Name:
RTN4-pS107
Product Number:
ab-pn983
Target Full Name: Reticulon-4
Target Alias: ASY; Foocen; KIAA0886; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; NOGO; Nogo protein; NSP; NSP-CL; Reticulon 4; Reticulon-4; Reticulon-5; RTN-x
Product Type Specific: RTN4 (Nogo) phosphosite-specific antibody
Antibody Code: PN983
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S107
Protein UniProt: Q9NQC3
Protein SigNET: RTN4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human RTN4
Antibody Immunogen Sequence: C(βA)ERQP(pS)WDP
Antibody Immunogen Description: Corresponds to amino acid residues E103 to P110. The effect of Nogo S107 phosphorylation is unclear. This is the major site of in vivo phosphorylation of RTN4 (Nogo) based on ≥369 mass spectrometry reports recorded in PhosphoSitePlus. RTN4 is known to be phosphorylated at this site in vitro by MAPKAPK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Scientific Background: RTN4 (Reticulon 4; Nogo) is a membrane protein primarily known for inhibiting axon regeneration in the central nervous system (CNS) and shaping the endoplasmic reticulum. RTN4 functions via Nogo receptors (RTN4R/NgR) on the surface of neurons or by interacting with other cell surface proteins. It features a highly conserved C-terminal reticulon domain (RHD) that helps shape and stabilize the endoplasmic reticulum (ER) membrane. Nogo isoforms are required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. Three isoforms (Nogo-A (RTN4A), -B (RTN4B), -C (RTN4C) regulate neurite branching, vascular remodeling, and inflammatory responses. Nogo-A, the largest isoform, is a key myelin-associated inhibitor (MAI) that restricts plastic changes and functional recovery after spinal cord injury. Nogo-A is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. It regulates neurite fasciculation, branching and extension in the developing nervous system. It is involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. It functions as a negative regulator of central nervous system angiogenesis. It inhibits spreading, migration and sprouting of primary brain microvascular endothelial cells (MVECs). Nogo-A is primarily found in the nervous system (neurons/oligodendrocytes). Nogo-B is widely expressed, particularly in endothelial and vascular smooth muscle cells, and regulates angiogenesis and inflammation. It is a modulator of vascular remodeling, promotes the migration of endothelial cells but inhibits the migration of vascular smooth muscle cells. It regulates endothelial sphingolipid biosynthesis with direct effects on vascular function and blood pressure. Nogo-B inhibits serine palmitoyltransferase, SPTLC1, the rate-limiting enzyme of the novo sphingolipid biosynthetic pathway, thereby controlling production of endothelial sphingosine-1-phosphate (S1P). It required to promote macrophage homing and functions such as cytokine/chemokine gene expression involved in angiogenesis, arteriogenesis and tissue repair. Nogo-C regulates cardiomyocyte apoptosis upon hypoxic conditions, and is expressed in brain, skeletal muscle, and adipose tissue. A fourth isoform, Nogo-D is testis-specific. Elevated levels of RTN4 are associated with Alzheimer’s, Parkinson’s, and multiple sclerosis. Nogo-B promotes tumour progression in certain cancers (e.g., nasopharyngeal) and plays a role in hepatic regeneration. This description may include information annotated by UniProt and/or Google AI.