Product Name:
Rictor-pT1135
Product Number:
ab-pn980
Target Full Name: Rapamycin-insensitive companion of mTOR
Target Alias: AVO3; KIAA1999; MAVO3; MGC39830; PIA; Pianissimo; RICTR; RPTOR independent companion of MTOR, complex 2
Product Type Specific: Rictor phosphosite-specific antibody
Antibody Code: PN980
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T1135
Protein UniProt: Q6R327
Protein SigNET: Rictor
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Rictor
Antibody Immunogen Sequence: IRTL(pT)EPS(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues I1131 to S1138. T1135 phosphorylation induces interaction with 14-3-3. This is the major in vivo phosphorylation sitesin Rictor (≥187 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Rictor is known to be phosphorylated at this site in vitro by Akt1 (PKBa), p70S6K (RPS6KB1), and SGK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.55 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus.
Related Product 1: Rictor S21 phosphorylation antibody (Cat. No.: AB-PN979)
Scientific Background: Rictor (Rapamycin-Insensitive Companion of mTOR) is a 200 kDa component of the mammalian target of rapamycin complex 2 (mTORC2), and belongs to the RICTOR family. It acts as a scaffold protein for a rapamycin-insensitive pathway that regulates cell growth, survival, cytoskeletal organization, and metabolism by activating AGC kinases like Akt1 (phosphorylates at Ser-473), SGK1 (phosphorylates at Ser-422), and PRKCA (phosphorylates at Ser-657). It is important for the assembly and stability of the mTORC2 complex, which includes mTOR, G-betaL, and mSIN1. Phosphorylation of Akt at Ser-473 is critical for full activation of Akt to promote cell survival and inhibiting apoptosis. Ser-473 phosphorylation of Akt may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1, which is a prerequisite for full activation. Rictor regulates the actin cytoskeleton, which affects cell migration and shape. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. It acts as a substrate for various kinases (e.g., GSK3) and its own phosphorylation by AGC kinases can regulate its activity. Rictor plays an essential role in embryonic growth and development. Rictor expression can be regulated by FoxO transcription factors. Unlike Raptor, which is part of the rapamycin-sensitive mTORC1 complex that regulates protein synthesis, Rictor is part of the mTORC2 complex, which is generally not inhibited by acute rapamycin treatment. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. Deregulation of Rictor-mTORC2 is associated with cancer progression and diabetes. It is involved in tumorigenesis, such as in hepatocellular carcinoma, where it aids cell migration and metabolism. This description may include information annotated by UniProt and/or Google AI.