Product Name:
PSEN1-pT354
Product Number:
ab-pn977
Target Full Name: Presenilin-1
Target Alias: AD3; AD3H; FAD; Presenilin-1 CTF12; PS1; PS-1; PSN1; PSNL1; S182; S182 protein
Product Type Specific: PSEN1 phosphosite-specific antibody
Antibody Code: PN977
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T354
Protein UniProt: P49768
Protein SigNET: PSEN1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PSEN1
Antibody Immunogen Sequence: PHRS(pT)PES(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues P350 to S357. T354 phosphorylation stimulates enzyme activity. This is a major phosphorylation site in vivo based on ≥24 mass spectrometry reports recorded in PhosphoSitePlus. PSEN1 is known to be phosphorylated at this site in vitro by CDK5, and DYRK1A.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.85 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse | Chicken.
Scientific Background: PSEN1 (Presenilin-1) is a 9-transmembrane domain-spanning protein that is the catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). It belongs to the peptidase A22A family. It requires the presence of the other members of the gamma-secretase complex for protease activity. It functions in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. It stimulates cell-cell adhesion via its interaction with CDH1, which stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin). Under conditions of apoptosis or calcium influx, it cleaves CDH1. This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and negatively regulates Wnt signaling. It is required for normal embryonic brain and skeleton development, and for normal angiogenesis. It mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is therefore involved in calcium homeostasis. It is involved in the regulation of neurite outgrowth. It is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity. It acts through the control of SYT7 presynaptic expression. It is detected in azurophile granules in neutrophils and in platelet cytoplasmic granules. As part of the gamma-secretase complex (along with Nicastrin, APH-1, and PEN-2), it resides in cell membranes, including the endoplasmic reticulum and Golgi. PSNE1 is expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes. Over 200 mutations in the PSEN1 gene are linked to familial Alzheimer's disease. Some mutations also cause frontotemporal dementia (FTD). Mutations in PSEN1 are the leading cause of early-onset Alzheimer's disease (EOAD), as they increase the production of neurotoxic amyloid-beta peptides. This description may include information annotated by UniProt and/or Google AI.