Product Name:
NGFR-pT293
Product Number:
ab-pn973
Target Full Name: Tumor necrosis factor receptor superfamily member 16
Target Alias: CD271; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Nerve growth factor receptor; NGF receptor; P75 ICD; P75NTR; TNFR superfamily, member 16; TNFRSF16; TNR16; Tumor necrosis factor receptor superfamily member 16
Product Type Specific: NGFR phosphosite-specific antibody
Antibody Code: PN973
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T293
Protein UniProt: P08138
Protein SigNET: NGFR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NGFR
Antibody Immunogen Sequence: PVNQ(pT)PPP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues P289 to P296. The effect of T293 phosphorylation is unknown. This is the major phosphosite in NGFR by mass spectrometry (≥6 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog.
Scientific Background: NGFR (Nerve Growth Factor Receptor; p75NTR; CD271, is a 75 kDa transmembrane glycoprotein and member of the tumour necrosis factor receptor (TNFR) superfamily. It binds with low affinity to all neurotrophins—including NGF, BDNF, NT-3, and NT-4/5—regulating neuronal survival, apoptosis, differentiation, and migration. It forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NT-3 with high affinity, and has much lower affinity for mature NGF and BDNF. The receptor consists of an extracellular domain with four cysteine-rich repeats, a single transmembrane domain, and a cytoplasmic death domain. NGFR lacks intrinsic tyrosine kinase activity but functions as a co-receptor with Trk receptors (TrkA, B, C), Sortilin, and LINGO1. It mediates both cell survival and death depending on the cellular context. It is crucial for nerve development, myelination, and plasticity. NGFR plays a role in the inactivation of RHOA, and the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells for glucose uptake in response to insulin. It is necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. It is expressed in the nervous system and various non-neuronal tissues, including mesenchymal stem cells and keratinocytes. Dysfunction is involved in neurodegenerative diseases (e.g., Alzheimer's), neural repair, and it acts as a marker for certain tumours, such as melanomas. This description may include information annotated by UniProt and/or Google AI.