Product Name:
NCAM1-pS784
Product Number:
ab-pn969
Target Full Name: Neural cell adhesion molecule 1
Target Alias: CD56; NCA11; NCAM; N-CAM 140; NCAM 180; NCAM-140; Neural cell adhesion molecule 1; Neural cell adhesion molecule 1 140 kDa isoform
Product Type Specific: NCAM1 phosphosite-specific antibody
Antibody Code: PN969
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S784
Protein UniProt: P13591
Protein SigNET: NCAM1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NCAM1
Antibody Immunogen Sequence: CSKDE(pS)KEP
Antibody Immunogen Description: Corresponds to amino acid residues S780 to P787. The effect of S784 phosphorylation is unknown. This is the major phosphosite in NCAM1 by mass spectrometry (34 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish.
Scientific Background: NCAM1 (Neural cell adhesion molecule 1; CD56) is a multifunctional transmembrane glycoprotein of the immunoglobulin superfamily that mediates neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, and synaptic plasticity in the nervous system. It is critical for neurodevelopment and immune cell regulation, specifically on NK cells and T lymphocyte subsets. Its adhesive properties are controlled by post-translational modifications, specifically polysialylation. It is also subject to proteolytic cleavage by metalloproteinases like ADAM17 and MMPs. Alternative splicing produces three main isoforms (NCAM-120, -140, -180) that differ in their intracellular domains. The molecule can be post-translationally modified by adding polysialic acid (PSA), which reduces adhesion and promotes cell plasticity. It is expressed on neurons, glial cells, skeletal muscle, and NK cells. It is linked to Alzheimer’s disease due to reduced synaptic levels and implicated in schizophrenia and bipolar disorder. It also acts as a receptor for rabies and Zika viruses. This description may include information annotated by UniProt and/or Google AI.