Product Name:
MBP-pY203
Product Number:
ab-pn968
Target Full Name: Myelin basic protein; Myelin A1 protein
Target Alias: Myelin A1 protein; Myelin membrane encephalitogenic protein
Product Type Specific: MBP phosphosite-specific antibody
Antibody Code: PN968
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y203
Protein UniProt: P02686
Protein SigNET: MBP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MBP
Antibody Immunogen Sequence: RTAH(pY)GSL(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R199 to L206. The effect of Y203 phosphorylation is unclear. This is the major in vivo phosphorylation sites in myelin basic protein (≥105 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Cow | Rabbit | Rat | Mouse | Chicken.
Related Product 1: MBP T229+T232 phosphorylation antibody (Cat. No.: AB-PN1024)
Related Product 2: MBP T232 phosphorylation antibody (Cat. No.: AB-PN558)
Scientific Background: MBP (Myelin basic protein) is a major protein component of the myelin sheath in the central nervous system (CNS), essential for maintaining the structure of myelin and insulating nerve fibers to increase action potential conduction velocity. It acts as a membrane adhesive protein, interacting with lipids in the myelin membrane and the cytoskeleton. As a key protein in oligodendrocyte function, it also acts as a biomarker for demyelination and CNS damage. It is an intrinsically disordered protein that acquires structure upon binding to lipids, and it exists in multiple isoforms generated by alternative splicing. The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, possibly as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. It induces T-cell proliferation. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Often considered a marker of active demyelination, autoantibodies against MBP are implicated in inflammatory diseases like multiple sclerosis. This description may include information annotated by UniProt and/or Google AI.