Product Name:
LRP4-pS1887
Product Number:
ab-pn967
Target Full Name: Low-density lipoprotein receptor-related protein 4
Target Alias: Leucine-rich repeat kinase 2; RIP7; PARK8; Dardarin; ROCO 2; RIPK7; augmented in rheumatoid arthritis 17
Product Type Specific: LRP4 phosphosite-specific antibody
Antibody Code: PN967
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1887
Protein UniProt: O75096
Protein SigNET: LRP4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human LRP4
Antibody Immunogen Sequence: ERRG(pS)LPD(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E1883 to D1890. The effect of LRP4 Ser-1887 phosphorylation is unclear. This is the major in vivo phosphorylation site in LRP4 (≥29 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). LRP4 is known to be phosphorylated at this site in vitro by CaMK2a.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse.
Related Product 1: LRP4 (Extracellular) expression antibody (Cat. No.: AB-NN286-1)
Related Product 2: LRP4 (Cytoplasmic) expression antibody (Cat. No.: AB-NN287-2)
Scientific Background: LRP4 (Low-density lipoprotein receptor-related protein 4) is a single-pass transmembrane receptor crucial for neuromuscular junction (NMJ) formation, bone development, and Wnt signaling regulation. It binds to neuronal agrin, activating the MuSK receptor (muscle-specific kinase), which triggers acetylcholine receptor (AChR) clustering and post-synaptic differentiation. Beyond the neuromuscular junction, LRP4 is essential for kidney development and limb development, particularly in branching morphogenesis. It binds and internalizes extracellular ligands for degradation by lysosomes. LRP4 serves as a receptor for [sclerostin] (SOST) and Dkk1, which are inhibitors of the Wnt signaling pathway, thereby regulating bone growth and remodeling. It is involved in the negative regulation of the canonical Wnt signalling pathway, being able to antagonize the LRP6-mediated activation of this pathway. It is a type I transmembrane protein with a large extracellular domain (containing LDL repeats, EGF-like repeats, and beta-propeller domains) and a short cytoplasmic tail. LRP4 mutations cause diseases like myasthenia gravis and congenital myasthenic syndrome. Autoantibodies targeting LRP4 are a known cause of seronegative myasthenia gravis, and genetic defects can lead to bone dysplasia. This description may include information annotated by UniProt and/or Google AI.