Product Name:
L1CAM-pY1176
Product Number:
ab-pn962
Target Full Name: Neural cell adhesion molecule L1
Target Alias: CAML; CAML1; CD171; CD171 antigen; HSAS; HSAS1; L1 cell adhesion molecule; MASA; MIC5; Neural cell adhesion molecule L1; S10; SPG1
Product Type Specific: L1CAM phosphosite-specific antibody
Antibody Code: PN962
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1176
Protein UniProt: P32004
Protein SigNET: L1CAM
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human L1CAM
Antibody Immunogen Sequence: TFGE(pY)RSL(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues T1172 to L1179. The effect of N-CAM L1 Y176 phosphorylation is unclear. This is a major site of in vivo phosphorylation in L1CAM based on ≥10 mass spectrometry reports documented in PhosphoSitePlus. L1CAM is known to be phosphorylated at this site in vitro by Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.65 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog.
Related Product 1: L1CAM T1172 phosphorylation antibody (Cat. No.: AB-PN961)
Scientific Background: L1CAM (Neural cell adhesion molecule L1; CD171) is a 200-220 kDa transmembrane glycoprotein from the immunoglobulin superfamily (L1/neurofascin/NgCAM family) that is crucial for nervous system development, including neuronal migration, axon growth and fasciculation, and synapse formation. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity. It mediates cell-cell adhesion and promotes cell motility. It functions through homophilic (L1-L1) and heterophilic (e.g., L1-integrin) binding. It features an extracellular domain (immunoglobulin-like and fibronectin-type III repeats), a transmembrane segment, and a conserved cytoplasmic domain. Mutations in L1CAM are associated with L1 syndrome (X-linked disorders (CRASH: Corpus callosum hypoplasia, Retardation, Aphasia, Spastic paraplegia, and Hydrocephalus), while its over-expression in various cancers (e.g., ovarian, colon, glioblastoma) drives invasion and chemoresistance. L1CAM is primarily expressed in the central and peripheral nervous systems. This description may include information annotated by UniProt and/or Google AI.