Product Name:
eIF4E-pS209
Product Number:
ab-pn956
Target Full Name: Eukaryotic translation initiation factor 4E
Target Alias: eIF-4F 25 kDa subunit;mRNA cap-binding protein
Product Type Specific: eIF4E phosphosite-specific antibody
Antibody Code: PN956
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S209
Protein UniProt: P06730
Protein SigNET: eIF4E
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human eIF4E
Antibody Immunogen Sequence: TKSG(pS)TTK(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues T205 to K212. Phosphorylation of S209 stimulates enzyme activity. It is one of the major phosphorylation sites in eIF4E with >5 reports by mass spectrometry listed in PhosphoSitePlus. eIF4E is known to be phosphorylated at this site in vitro by Mnk1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Chicken | Frog | Zebra fish.
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Scientific Background: eIF4E (Eukaryotic translation initiation factor 4E) is a critical 20-25 kDa protein in the eukaryotic initiation factor 4E family that binds the 7-methylguanosine cap at the 5′ end of mRNA, which is a rate-limiting step in cytoplasmic protein synthesis. It has a specialized structure with a ventral surface for cap-binding and a dorsal surface that interacts with 4E-BPs and eIF4G. As a key component of the eIF4F complex (with eIF4G and eIF4A), it initiates translation and is involved in mRNA export from the nucleus. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. Because eIF4E is the least abundant translation initiation factor, it acts as a bottleneck, regulating the rate of translation. Its activity is controlled by 4E-binding proteins (4E-BPs) that compete with eIF4G for binding, and by phosphorylation (Ser-209) catalyzed by Mnk1/2 kinases. It plays a role in synaptic plasticity, with dysregulation associated with autism and other neurodevelopmental disorders. Overexpression of eIF4E is linked to malignant transformation (e.g., breast, prostate, lung, colon) through promotion of the translation of oncogenic mRNAs involved in proliferation, survival, and angiogenesis. This description may include information annotated by UniProt and/or Google AI.