Product Name:
Dvl1-pS93
Product Number:
ab-pn955
Target Alias: Dishevelled, dsh homolog 1 (Drosophila); Dishevelled, dsh homolog 1 (Drosophila), isoform CRAc; Dishevelled-1; DSH homolog 1; DSH homolog 1-like; DSH homologue 1; DVL; Putative uncharacterized protein DVL1; Segment polarity protein dishevelled homolog DVL-1-like; Segment polarity protein dishevelled homologue DVL-1
Product Type Specific: Dvl1 phosphosite-specific antibody
Antibody Code: PN955
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S93
Protein UniProt: O14640
Protein SigNET: Dvl1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Dvl1
Antibody Immunogen Sequence: CSDAG(pS)QGT
Antibody Immunogen Description: Corresponds to amino acid residues S89 to T96.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.15 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken.
Scientific Background: Dvl1 (Segment polarity protein dishevelled homolog DVL-1) is an inhibitor protein that belongs to the Dishevelled family and contains three conserved domains: an N-terminal DIX domain, a central PDZ domain, and a C-terminal DEP domain. It is essential for development, and regulates cell proliferation, differentiation, and neuroblast specification. It binds directly to Frizzled receptors and it plays a role both in canonical and non-canonical Wnt signalling. It participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and recruits Axin to the membrane, which inhibits the beta-catenin destruction complex, allowing beta-catenin to accumulate. Its function in the signal transduction pathways mediated by multiple Wnt genes. It is required for LEF1 activation upon WNT1 and WNT3A signalling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Dvl1 is typically found in the cytoplasm, it translocates to the plasma membrane upon Wnt activation, and can also be found in the nucleus. It is expressed in both fetal and adult tissues, with high levels in the brain, skeletal muscle, pancreas, and heart. Dvl1 is critical for proper development. Mutations are linked to autosomal dominant/recessive Robinow syndrome, which affects skeletal and facial development. Overexpression of Dvl1 is linked to poor prognosis in various cancers (e.g., non-small cell lung cancer), and its dysfunction is implicated in neurodegenerative diseases. This description may include information annotated by UniProt and/or Google AI.