Product Name:
DOK7-pY405
Product Number:
ab-pn953
Target Full Name: Docking protein 7
Target Alias: Downstream of tyrosine kinase 7
Product Type Specific: DOK7 phosphosite-specific antibody
Antibody Code: PN953
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y405
Protein UniProt: Q18PE1
Protein SigNET: DOK7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DOK7
Antibody Immunogen Sequence: C(βA)RAH(pY)DTP
Antibody Immunogen Description: Corresponds to amino acid residues R402 to P408. The effect of Y405 phosphorylation is unclear. This is the major in vivo phosphorylation site in Dok7 (≥4 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Mouse.
Related Product 1: DOK3 Y398 phosphorylation antibody (Cat. No.: AB-PN508)
Related Product 2: DOK7 Y395 phosphorylation antibody (Cat. No.: AB-PN952)
Scientific Background: Dok7 is an activator and cytoplasmic adapter protein essential for forming and maintaining the neuromuscular junction (NMJ). It is likely the muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. It acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. It induces autophosphorylation of MUSK. It contains an N-terminal pleckstrin homology (PH) domain and a phosphotyrosine-binding (PTB) domain, which are critical for binding and activating the MuSK receptor. It works with agrin and LRP4 to establish neuromuscular synapses. Dok7 is preferentially expressed in thigh skeletal muscle, diaphragm and heart, and not evident in the liver or spleen. Mutations in the Dok7 gene lead to Dok7 congenital myasthenic syndrome, a neuromuscular disorder characterized by limb-girdle weakness. Rare cases of fetal akinesia deformation sequence, which is typically fatal before birth, have also been linked to DOK7 mutations. This description may include information annotated by UniProt and/or Google AI.