Product Name:
DAG1-pT790
Product Number:
ab-pn947
Target Full Name: Dystroglycan
Target Alias: Alpha-dystroglycan; Beta-dystroglycan; Dystrophin-associated glycoprotein 1
Product Type Specific: DAG1 phosphosite-specific antibody
Antibody Code: PN947
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T790
Protein UniProt: Q14118
Protein SigNET: DAG1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DAG1
Antibody Immunogen Sequence: DQA(pT)FIKKGC
Antibody Immunogen Description: Corresponds to amino acid residues D787 to K795. The effect of T790 phosphorylation is unclear. This is the major site of in vivo phosphorylation with ≥34 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Sea urchin.
Scientific Background: DAG1 (Dystroglycan 1) is a multifunctional protein that ifunctions in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan is an extracellular peripheral glycoprotein that acts as a receptor for both extracellular matrix proteins containing laminin-G domains. It is also a receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Beta-dystroglycan is a transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. It acts as a cell adhesion receptor in both muscle and non-muscle tissues, and as a receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. It also functions in cell adhesion-mediated signaling and implicated in control of cell polarity. Alpha-dystroglycan acts as a receptor for lassa virus and lymphocytic choriomeningitis virus glycoprotein and class C new-world arenaviruses. Alpha-dystroglycan acts as a Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy, but only in the presence of the G-domain of LAMA2. It is primarily expressed in skeletal and heart muscle fibers, as well as peripheral nerves and neuronal tissues as well as keratinocytes and fibroblasts. In epidermal tissue, it located to the basement membrane. Mutations affecting DAG1 or its glycosylation cause congenital muscular dystrophies known as dystroglycanopathies, which lead to muscle fragility, necrosis, and in some cases, brain abnormalities. This description may include information annotated by UniProt and/or Google AI.