Product Name:
CRMP2-pY499
Product Number:
ab-pn944
Target Full Name: Dihydropyrimidinase-related protein 2
Target Alias: Collapsin response mediator protein 2; CRMP2; DHPRP2; Dihydropyrimidinase related protein-2; Dihydropyrimidinase-like 2; DPYL2; DRP2; DRP-2; N2A3; TOAD-64; Turned on after division, 64 kDa protein; ULIP 2 protein; ULIP2
Product Type Specific: CRMP2 (DPYSL2) phosphosite-specific antibody
Antibody Code: PN944
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y499
Protein UniProt: Q16555
Protein SigNET: CRMP2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CRMP2
Antibody Immunogen Sequence: PRGL(pY)DGPVC
Antibody Immunogen Description: Corresponds to amino acid residues P495 to V494. T509 phosphorylation regulates molecular association. This is the major phosphorylation site in CRMP2 (≥333 reports by mass spectrometry in PhosphoSitePlus). CRMP2 is known to be phosphorylated at this site in vitro by Fer.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish.
Related Product 1: CRMP2 T509 phosphorylation antibody (Cat. No.: AB-PN814)
Related Product 2: CRMP2 T514 phosphorylation antibody (Cat. No.: AB-PN943)
Scientific Background: CRMP2 (Dihydropyrimidinase-related protein 2; DPYSL2) is a ubiquitous, microbule-binding protein that belongs to the metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family. It functions in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. It promotes axonogenesis by enhancing microtubule formation and regulating neurotransmitter release. CRMP2 binds to heterodimers of tubulin and promotes microtubule assembly, which is crucial for axon guidance and neurite outgrowth. It also acts as a cargo adapter for trafficking and regulates CaV2.2 (calcium channel) activity. It is necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. It may also play a role in endocytosis. It is unclear if it has dihydropyrimidinase enzymatic activity. Its activity is tightly regulated through post-translational modifications, including phosphorylation by kinases like GSK-3β CDK5, and Rho-kinase (ROCK), which typically inhibits its activity by reduces its affinity for tubulin (thereby inhibiting axon growth), and through altered Calmodulin (CaM) binding. Dysfunction in CRMP2 is linked to neurodegenerative diseases (Alzheimer’s), neurological disorders (neuropathic pain), and psychiatric conditions (schizophrenia). Hyperphosphorylated CRMP2 is found in neurofibrillary tangles. This description may include information annotated by UniProt and/or Google AI.