Product Name:
CNTN1-pY742
Product Number:
ab-pn942
Target Full Name: Contactin-1
Target Alias: Glycoprotein gp135; Neural cell surface protein F3
Product Type Specific: CNTN1 phosphosite-specific antibody
Antibody Code: PN942
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y742
Protein UniProt: Q12860
Protein SigNET: CNTN1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CNTN1
Antibody Immunogen Sequence: NNFG(pY)IVA(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues N738 to A745. The effect of Y742 phosphorylation is unclear. This is a minor site of in vivo phosphorylation in CNTN1 based on ≥1 mass spectrometry report recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Zebra fish.
Scientific Background: CNTN1 (Contactin-1) belongs to the immunoglobulin superfamily, Contactin family. Contactins mediate cell surface interactions during nervous system development. It features six Ig-like C2-type domains and four Fibronectin type-III domains. CNTN1 is involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. It participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. CNTN1 interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. It is strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression of CNTN1 are seen in lung, pancreas, kidney and skeletal muscle. Autoantibodies against CNTN1 can produce conditions like chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). Overexpression of CNTN1 is associated with cancer cell invasion, migration, and metastasis (e.g., in lung and gastric cancers), This description may include information annotated by UniProt and/or Google AI.