Product Name:
CLTC-pY634
Product Number:
ab-pn941
Target Full Name: Clathrin heavy chain 1
Target Alias: Clathrin heavy chain; Clathrin, heavy chain; Clathrin, heavy polypeptide (Hc); CLH; CLH1; CLH17; CLH-17; CLTCL2; Hc; KIAA0034
Product Type Specific: CLTC phosphosite-specific antibody
Antibody Code: PN941
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y634
Protein UniProt: Q00610
Protein SigNET: CLTC
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CLTC
Antibody Immunogen Sequence: FTDL(pY)DIK(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F630 to K637. The effect of T634 phosphorylation is unclear. This is one of the major sites of in vivo phosphorylation of CLTC (≥x1083 reports by mass spectrometry in PhosphoSitePlus). CLTC is known to be phosphorylated at this site in vitro by ZAP70.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Nematode worm | Sea urchin .
Scientific Background: CLTC (Clathrin heavy chain 1) belongs to the clathrin heavy chain family, and is the major 190 kDa protein of the polyhedral coat of coated pits and vesicles. The protein features three heavy chains (1,675 amino acids) and three light chains, forming a 3-legged structure called a triskelion. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. It acts as component of the TACC3/ch-TOG/clathrin complex and is proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. It functions in early autophagosome formation. Although it is ubiquitously expressed, it is abundant in the brain. This description may include information annotated by UniProt and/or Google AI.