Product Name:
CACNB1-pT499
Product Number:
ab-pn938
Target Full Name: Voltage-dependent L-type calcium channel subunit beta-1
Target Alias: CAB1; CACB1; CACNLB1; Calcium channel voltage-dependent subunit beta 1; Calcium channel, voltage-dependent, beta 1; Calcium channel, voltage-dependent, beta 1 subunit; Voltage-dependent L-type calcium channel beta-1
Product Type Specific: CACNB1 phosphosite-specific antibody
Antibody Code: PN938
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T499
Protein UniProt: Q02641
Protein SigNET: CACNB1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CACNB1
Antibody Immunogen Sequence: SRQD(pT)FDA)βA)C
Antibody Immunogen Description: Corresponds to amino acid residues S495 to A502. The effect of T499 phosphorylation is unclear. This is the major in vivo phosphorylation sites in CACNB1 (≥29 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken.
Scientific Background: CACNB1 (Voltage-dependent L-type calcium channel subunit beta-1) is the regulatory subunit of L-type calcium channels and belongs to the calcium channel beta subunit family. It controls the activity of L-type calcium channels that contain CACNA1A as pore-forming subunit. It regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit and increases the presence of the channel complex at the cell membrane. It is required for functional expression L-type calcium channels that contain CACNA1D as pore-forming subunit. It regulates the activity of L-type calcium channels that contain CACNA1B as pore-forming subunit. Isoform 1 and isoform 3 are expressed in brain, heart, spleen, central nervous system and neuroblastoma cells. Isoform 2 is also expressed in skeletal muscle. Loss of this protein has been linked to severe muscle mass reduction. This description may include information annotated by UniProt and/or Google AI.