Product Name:
AP3B2-pS272
Product Number:
ab-pn937
Target Full Name: AP-3 complex subunit beta-2
Target Alias: Beta3B-adaptin; Clathrin assembly protein complex 3 beta-2 large chain; Neuron-specific vesicle coat protein beta-NAP
Product Type Specific: AP3B2 phosphosite-specific antibody
Antibody Code: PN937
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S272
Protein UniProt: Q13367
Protein SigNET: AP3B2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AP3B2
Antibody Immunogen Sequence: CAFYG(pS)EED
Antibody Immunogen Description: Corresponds to amino acid residues A268 to D275. The effect of S272 phosphorylation is unclear. This is the major in vivo phosphoryation sites with ≥ 23 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.55 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Chicken | Zebra fish.
Related Product 1: AP2B1 Y276 phosphorylation antibody (Cat. No.: AB-PN934)
Related Product 2: AP2M1 T156 phosphorylation antibody (Cat. No.: AB-PN935)
Related Product 3: AP3B1 S276 phosphorylation antibody (Cat. No.: AB-PN936)
Scientific Background: AP3B2 (AP-3 complex subunit beta-2) is a subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3), which belongs to the adaptor complexes large subunit family. It functions in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. In neuronal development, it is involved in proper axonal morphology and the positioning of photoreceptor synapses in the retina. Isoform 1 expression is specific to nervous system, where it is found in the nerve terminal and cell body, and is associated with nerve-terminal vesicles. Its expression is also seen in Purkinje cells, cortical neurons, neuroectodermal tumours and graded in cerebral cortex (deeper layers exhibit stronger expression). Isoform 2 is expressed at high levels in brain and testis. Autosomal-recessive mutations in the AP3B2 gene cause Developmental and Epileptic Encephalopathy 48 (DEE48), which includes symptoms like severe epilepsy, developmental delay, and often optic atrophy. Autoantibodies against AP3B2 are associated with a rare form of autoimmune cerebellar/sensory ataxia, which presents with gait instability. This description may include information annotated by UniProt and/or Google AI.