Product Name:
AP2M1-pT156
Product Number:
ab-pn935
Target Full Name: AP-2 complex subunit mu
Target Alias: A2M1; adaptor-related protein complex 2, mu 1 subunit; AP-2 mu 2 chain; AP2M1; AP50; CLAPM1; clathrin adaptor complex AP2, mu subunit; Clathrin assembly protein complex 2 medium chain; clathrin coat adaptor protein AP50; Clathrin coat Assembly protein AP50; Clathrin coat associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; HA2 50 kDa subunit; KIAA0109; mu2; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein
Product Type Specific: AP2M1 phosphosite-specific antibody
Antibody Code: PN935
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T156
Protein UniProt: Q96CW1
Protein SigNET: AP2M1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AP2M1
Antibody Immunogen Sequence: SQV(pT)GQIGC
Antibody Immunogen Description: Corresponds to amino acid residues S153 to G160. T156 phosphorylation induces activity. This is the major site of phosphorylation of AP2M1 with ≥ 61 mass spectrometry reports in PhosphoSite Plus. AP2M1 is known to be phosphorylated at this site in vitro by LRRK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.55 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Nematode worm.
Related Product 1: AP2B1 Y276 phosphorylation antibody (Cat. No.: AB-PN934)
Related Product 2: AP3B1 S276 phosphorylation antibody (Cat. No.: AB-PN936)
Related Product 3: AP3B2 S272 phosphorylation antibody (Cat. No.: AB-PN937)
Scientific Background: AP2M1 (AP-2 complex subunit mu) is a component of the adaptor protein complex 2 (AP-2). These adaptor protein complexes play a role in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins by recognizing Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1. This description may include information annotated by UniProt and/or Google AI.