Product Name:
AP2B1-pY276
Product Number:
ab-pn934
Target Full Name: AP-2 complex subunit beta
Target Alias: Adapter-related protein complex 2 beta 1 subunit; adaptin, beta; adaptor-related protein complex 2, beta 1 subunit; ADTB2; AP105B; beta-adaptin; CLAPB1; clathrin assembly protein complex 2 beta large chain; plasma membrane adaptor HA2/AP2 adaptin beta subunit
Product Type Specific: AP2B1 phosphosite-specific antibody
Antibody Code: PN934
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y276
Protein UniProt: P63010
Protein SigNET: AP2B1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AP2B1
Antibody Immunogen Sequence: KDSD(pY)YNM(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues K272 to M279. The effect of Y276 phosphorylation is unclear. However, Y277 is known to affect protein interactions. This is one of the two major in vivo phosphoryation sites with ≥ 50 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: AP2M1 T156 phosphorylation antibody (Cat. No.: AB-PN935)
Related Product 2: AP3B1 S276 phosphorylation antibody (Cat. No.: AB-PN936)
Related Product 3: AP3B2 S272 phosphorylation antibody (Cat. No.: AB-PN937)
Scientific Background: AP2B1 (AP-2 complex subunit beta) is a component of the adaptor protein complex 2 (AP-2). These adaptor protein complexes play a role in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly. This description may include information annotated by UniProt and/or Google AI.