Product Name:
AKAP12-pS696
Product Number:
ab-pn933
Target Full Name: A-kinase anchor protein 12
Target Alias: A kinase (PRKA) anchor protein 12; AKA12; AKAP250; A-kinase anchor protein 250 kDa; Gravin; Myasthenia gravis autoantigen; Myasthenia gravis autoantigen gravin; RP11-351K16.7-001; SSeCKS
Product Type Specific: AKAP12 phosphosite-specific antibody
Antibody Code: PN933
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S696
Protein UniProt: Q02952
Protein SigNET: AKAP12
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AKAP12
Antibody Immunogen Sequence: CARRG(pS)SSDE
Antibody Immunogen Description: Corresponds to amino acid residues A692 to E700. Phosphorylation of S696 inhibits receptor desensitization. AKAP12 is known to be phosphorylated at this site in vitro by PKACA .
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Related Product 1: AKAP12 S627+pS629 phosphorylation antibody (Cat. No.: AB-PN1009)
Scientific Background: AKAP12 (A-kinase anchoring protein-12) mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC) isoforms as well as Src-family kinases, and calmodulin, enabling crosstalk between these pathways. This adaptor/scaffold protein is expressed in endothelial cells, astrocytes, cultured fibroblasts and osteosarcomas, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. It plays a crucial role in maintaining tissue integrity and the blood-brain barrier.It is often silenced in cancers (colon, liver, prostate) through promoter hypermethylation, leading to increased invasion and metastasis. AKAP12 regulates actin-cytoskeleton reorganization, promoting cell flattening and reducing migratory behavior. Loss of AKAP12 leads to hyperactivation of PKC and increased tumour growth, while its regulation is also implicated in neurological diseases like Alzheimer’s and ischemic injury. This description may include information annotated by UniProt and/or Google AI.