Product Name:
GFAP-pS13
Product Number:
ab-pn932
Target Full Name: Glial fibrillary acidic protein
Target Alias: Intermediate filament protein; Astrocyte; gfapl; DKFZp459C0729; MGC139638; FLJ45472; AI836096; GFAP
Product Type Specific: GFAP cytoskeletal protein phosphosite-specific antibody
Antibody Code: PN932
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S13
Protein UniProt: P14136
Protein SigNET: GFAP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human GFAP
Antibody Immunogen Sequence: CAARR(pS)YVS
Antibody Immunogen Description: Corresponds to amino acid residues A9 to S16. Phosphorylation of GFAP induces interaction with Fascin. This has been moderately reported as phosphorylated in ≥5 mass spectrometry studies in PhosphoSitePlus. GFAP is known to be phosphorylated at this site in vitro by AurB, PKACA, and ROCK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse.
Related Product 1: GFAP expression antibody (Cat. No.: AB-NN260-1)
Related Product 2: GFAP R416WT expression antibody (Cat. No.: AB-NN260-2)
Related Product 3: GFAP expression antibody (Cat. No.: AB-NN260-3)
Scientific Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. It features a central rod domain, an amino-terminal head, and a carboxyterminal tail. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. GFAP regulates astrocyte motility, shape, and structure, contributing to the cytoskeleton. It also plays a role in astrocyte reactivity, responding to CNS injury. It is mainly found in astrocytes and ependymal cells in the brain and spinal cord, but it is also expressed in some peripheral tissues, such as Leydig cells in the testis and some cells in the kidney. Antibodies to GFAP have been detected in individuals with dementia. GFAP is highly upregulated in response to injury (e.g., ischemia, trauma) and neurological diseases, such as Alzheimer's Disease and NMOSD. This description may include information annotated by UniProt and/or Google AI.