Product Name:
AChE-pY164
Product Number:
ab-pn931
Target Full Name: Acetylcholinesterase
Target Alias: ACES; Acetylcholinesterase (Yt blood group); ARACHE; EC 3.1.1.7; N-ACHE; YT
Product Type Specific: AChE phosphosite-specific antibody
Antibody Code: PN931
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y164
Protein UniProt: P22303
Protein SigNET: AChE
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AChE
Antibody Immunogen Sequence: CSLDV(pY)DGR
Antibody Immunogen Description: Corresponds to amino acid residues S160 to R167. Phosphorylation of Y164 of acetylcholinesterase stimuates enzyme activity. This has not been reported as an in vivo phosphorylation site based on high throughput mass spectrometry studies recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Honey bee | Nematode worm.
Scientific Background: ACACB (acetyl-CoA carboxylase-2; ACC2) is a mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA ((ATP + acetyl-CoA + HCO3- <=> ADP + phosphatse + malonyl-CoA)) and plays a central role in fatty acid metabolism, the first and rate-limiting step of de novo fatty acid biosynthesis. The two step reaction starts with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. It features a central biotin carboxyl carrier (BCC) domain, an N-terminal biotin carboxylase (BC) domain, and a C-terminal carboxyl transferase (CT) domain. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, it is thought to negatively regulate fatty acid oxidation. Unlike its cytosolic isoform ACACA, ACACB is located on the outer mitochondrial membrane, anchored by a unique N-terminal sequence. Along with its cytosolic isozyme ACACA, it promotes lipid storage. It is inhibited by long-chain fatty acyl CoAs and activated by citrate. It is widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon. Isoform 3 is expressed highly in adipose tissue, and at lower levels in heart, liver, skeletal muscle and testis. This description may include information annotated by UniProt and/or Google AI.