Product Name:
VCP-pT56
Product Number:
ab-pn929
Target Full Name: Transitional endoplasmic reticulum ATPase
Target Alias: 15S Mg(2+)-ATPase p97 subunit; IBMPFD; P97; TER ATPase; TERA; Valosin containing protein; Valosin-containing protein
Product Type Specific: VCP phosphosite-specific antibody
Antibody Code: PN929
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T56
Protein UniProt: P55072
Protein SigNET: VCP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human VCP
Antibody Immunogen Sequence: RGD(pT)VLLKGC
Antibody Immunogen Description: Corresponds to amino acid residues R53 to G61. The effect of T56 phosphorylation is unclear. This is a minor in vivo site of phosphorylation of VCP based on ≥2 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Thale cress | Budding yeast.
Related Product 1: VCP S37 phosphorylation antibody (Cat. No.: AB-PN925)
Related Product 2: VCP T316 phosphorylation antibody (Cat. No.: AB-PN927)
Related Product 3: VCP T509 phosphorylation antibody (Cat. No.: AB-PN928)
Related Product 4: VCP S702 phosphorylation antibody (Cat. No.: AB-PN926)
Related Product 5: VCP T761 phosphorylation antibody (Cat. No.: AB-PN930)
Related Product 6: VCP Y805 phosphorylation antibody (Cat. No.: AB-PN1007)
Scientific Background: VCP (Valosin-containing protein) is a transitional endoplasmic reticulum ATPase that belongs to the AAA ATPase family. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It functions in vesicle transport and fusion, and in the formation of the transitional endoplasmic reticulum (tER). tER contains ER exit sites involved in the transfer of membranes from the endoplasmic reticulum to the Golgi apparatus. Vesicle budding from the tER is an ATP-dependent process. In the NPLOC4-UFD1-VCP complex, VCP binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex also regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It controls the E3 ubiquitin-protein ligase activity of RNF19A. As a component of the VCP-AMFR complex, it participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. It is involved in ER stress-induced preemptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the ER and reroutes them to the cytosol for proteasomal degradation. It functions in the regulation of the stress granule (SGs) clearance process upon arsenite-induced response. It is also nvolved in the DNA damage response by recruiting to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promoting the recruitment of TP53BP1 at DNA damage sites. It is recruited to stalled replication forks by SPRTN, and it may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. VCP is needed for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. It is required for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. It functions as a negative regulator of type I interferon production. Mutation in VCP have been linked to amyotrophic lateral sclerosis. This description may include information annotated by UniProt and/or Google AI.