Product Name:
SQSTM1-pT269+pS272
Product Number:
ab-pn909
Target Full Name: Sequestosome-1
Target Alias: EBI3-associated protein of 60 kDa; ORCA; Osi; SQSTM 1; ZIP 3; A170; SQSTM1; Ubiquitin binding protein p62; Protein kinase C-zeta-interacting protein; FTDALS3; Ubiquitin-binding protein p62; PKC-zeta-interacting protein; EBIAP; Sqstm1; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa; EBI3 associated protein p60; EBI3 associated protein of 60 kDa; SQSTM_HUMAN; OSF-6; Paget disease of bone 3; p62B; p60; PDB 3; EBI 3 associated protein p60; MGC127197; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBI 3 associated protein of 60 kDa; ZIP3; STAP; p62; Sequestosome 1; OSIL; STONE14; Phosphotyrosine independent ligand for the Lck SH2 domain p62; PDB3; Oxidative stress induced like; ZIP
Product Type Specific: SQSTM1 adapter/scaffold protein phosphosite-specific antibody
Antibody Code: PN909
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T269+S272
Protein UniProt: Q13501
Protein SigNET: SQSTM1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SQSTM1
Antibody Immunogen Sequence: SRL(pT)PV(pS)PESSC
Antibody Immunogen Description: Corresponds to amino acid residues S266 to S276. The effects of SQSTM1 T269 and S272 phosphorylation are unclear. These are two major sites of in vivo phosphorylation of SQSTM1 (≥208 and ≥335 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). SQSTM1 is known to be phosphorylated in vitro at T269 and S272 by CDK1 (CDC2), and p38d MAPK (MAPK13).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Related Product 1: SQSTM1 expression antibody (Cat. No.: AB-NN375-1)
Related Product 2: SQSTM1 S403 phosphorylation antibody (Cat. No.: AB-PN908)
Related Product 3: SQSTM1 T339+S342 phosphorylation antibody (Cat. No.: AB-PN910)
Scientific Background: SQSTM1 (Sequestome 1 (p62)) is a ubiquitin binding protein that interacts with ubiqutin, providing a scaffold for several signalling proteins and triggering degradation of proteins throught the proteasome or lysosome. It functions as a bridge between polyubiquitinated cargo and autophagosomes. It regulates activation of the nuclear factor kappa-B (NF-kB) signalling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. SQSTM1 also binds autophagosomal membrane protein LC3/ATG8. Mutations in the SQSTM1 gene result in sporadic and familial Paget disease of bone, and have been linked with amyotrophic lateral sclerosis (ALS). This description may include information annotated by UniProt and/or Google AI.