Product Name:
SOD1-pS108
Product Number:
ab-pn903
Target Full Name: Superoxide dismutase [Cu-Zn]
Target Alias: Superoxide dismutase 1; SOD; SOD1 exposed dimer interface
Product Type Specific: SOD1 (Cu-Zn) superoxide dismutase pan-specific antibody
Antibody Code: PN903
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S108
Protein UniProt: P00441
Protein SigNET: SOD1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SOD1
Antibody Immunogen Sequence: VISL(pS)GDHC
Antibody Immunogen Description: Corresponds to amino acid residues V104 to H111. The effect of S108 phosphorylation is unclear. This is a major site of in vivo phosphorylation of SOD1 (≥20 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Rat | Mouse | Chicken.
Related Product 1: SOD (Cu/Zn) expression antibody (Cat. No.: AB-NN098-2)
Related Product 2: SOD (Cu/Zn) expression antibody (Cat. No.: AB-NN098-3)
Related Product 3: SOD1 S99 phosphorylation antibody (Cat. No.: AB-PN904)
Related Product 4: SOD1 T40 phosphorylation antibody (Cat. No.: AB-PN905)
Related Product 5: SOD1 (EDI) expression antibody (Cat. No.: AB-NN098-4)
Related Product 6: SOD1 (UβB) expression antibody (Cat. No.: AB-NN098-5)
Related Product 7: SOD (EC) expression antibody (Cat. No.: AB-NN328-1)
Related Product 8: SOD (EC) expression antibody (Cat. No.: AB-NN328-2)
Related Product 9: SOD (Mn) expression antibody (Cat. No.: AB-NN068-1)
Related Product 10: SOD (Mn) expression antibody (Cat. No.: AB-NN068-2)
Scientific Background: SOD1 (cytoplasmic copper-zinc superoxide dismutase) serves as an important antioxidant that catalyzes the conversion of the toxic reactive oxygen species superoxide radical O2ˉ anion into oxygen (O2) and hydrogen peroxide (H2O2), which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). It is an intracellular metalloenzyme that is found in almost every cell in the body (2). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in amyotrophic lateral sclerosis (ALS). Over 180 different mutations in SOD1 have been linked to ALS. Conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UβB) (SPC-205D) which detects an unfolded beta barrel (UβB) of SOD1 This description may include information annotated by UniProt and/or Google AI.