Product Name:
OPTN-pS526+pS528
Product Number:
ab-pn897
Target Full Name: Optineurin
Target Alias: FIP2; GLC1E; HIP7; HYPL; NRP; Optic neuropathy-inducing protein; TFIIIA-INTP
Product Type Specific: OPTN phosphosite-specific antibody
Antibody Code: PN897
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S526+S528
Protein UniProt: Q96CV9
Protein SigNET: OPTN
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human OPTN
Antibody Immunogen Sequence: CGAR(pT)(pS)DSD
Antibody Immunogen Description: Corresponds to amino acid residues G522 to D529. The effects of S256 and S258 phosphorylation are unclear. These are minor in vivo phosphorylation sites in OPTN based on mass spectrometry (≥19 and ≥6 reports, respectively, in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.85 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Related Product 1: OPTN S177 phosphorylation antibody (Cat. No.: AB-PN895)
Related Product 2: OPTN S342 phosphorylation antibody (Cat. No.: AB-PN896)
Scientific Background: OPTN (Optineurin) is an inhibitory protein that plays an important role in the maintenance of the Golgi complex and Golgi ribbon formation, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. It recruits the protein kinase TBK1 at the Golgi apparatus, and promotes its trans-phosphorylation and activation after RLR or TLR3 stimulation, such a during stimulation of the innate immune response following viral infection. The activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. It also functions with a neuroprotective role in the eye and optic nerve, likely by regulating membrane trafficking and cellular morphogenesis via a Rab8 and hungtingtin (HD) containing complex. It mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR). It also controls Rab8 recruitment to tubules emanating from the endocytic recycling compartment. As an autophagy receptor, it interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. It targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. It may be a cellular target protein for adenovirus E3 14.7 and Bluetongue virus protein NS3 to inhibit innate immune responses. OPTN is present in aqueous humor of the eye and highly expressed in trabecular meshwork. It is also found in nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta. Mutations in OPTN have also be linked to amyotrophic lateral sclerosis (ALS). This description may include information annotated by UniProt and/or Google AI.