Product Name:
NF-M-pS620
Product Number:
ab-pn893
Target Full Name: Neurofilament medium polypeptide
Target Alias: 160 kDa neurofilament protein; NEF3; Neurofilament 3; Neurofilament triplet M protein; Neurofilament, medium polypeptide; NF-M
Product Type Specific: NF-M (NEFM) phosphosite-specific antibody
Antibody Code: PN893
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S620
Protein UniProt: P07197
Protein SigNET: NF-M
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NF-M
Antibody Immunogen Sequence: C(βA)VPK(pS)PVEE
Antibody Immunogen Description: Corresponds to amino acid residues V617 to E624. The effect of NFM Ser-620 phosphorylation is unclear. This is one of the major in vivo phosphosites in NFM by mass spectrometry (≥53 reports recorded in PhosphoSitePlus). NF-M is known to be phosphorylated at this site in vitro by PKACa (PRKACA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse | Platypus | Chicken.
Related Product 1: NF-L expression antibody (Cat. No.: AB-NN582-1)
Related Product 2: NF-M Y320 phosphorylation antibody (Cat. No.: AB-PN894)
Related Product 3: NF-H expression antibody (Cat. No.: AB-NN583-1)
Related Product 4: NF-H S532 phosphorylation antibody (Cat. No.: AB-PN970)
Related Product 5: N-FH S606 phosphorylation antibody (Cat. No.: AB-PN971)
Related Product 6: N-FH S648 phosphorylation antibody (Cat. No.: AB-PN972)
Scientific Background: NF-M (NEFM; neurofilament medium chain) is a 160 kDa cytoskeletal protein that belongs to the intermediate filament family and a structural protein in axons. Neurofilaments usually contain three intermediate filament proteins, L, M, and H, all of which are involved in the maintenance of neuronal caliber. It co-assembles with NF-L and NF-H to provide structural integrity, particularly controlling axonal radial growth and maintaining spacing between filaments. NF-M likely cooperates with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. NF-M consists of a core filament structure and a long C-terminal tail domain that projects outward, forming cross-bridges with other neurofilaments, actin, and microtubules. Its tail domain is critical for expansion of axon diameter, and its phosphorylation level regulates this process. Phosphorylation of the C-terminal tail of NF-M regulates its interaction with other proteins, including the dopamine D1 receptor. NF-M is found in large myelinated axons and neuronal cytoplasm. It can become elevated in the blood and cerebrospinal fluid as markers of neuronal damage in neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). While neurofilament light (NEFL) is the most studied, elevated NFM levels in spinal cord tissue and plasma have been observed in ALS patients. NF-M is also biomarker for other neurodegenerative diseases like multiple sclerosis, and dementia. This description may include information annotated by UniProt and/or Google AI.