Product Name:
MATR3-pS188
Product Number:
ab-pn883
Target Full Name: Matrin-3
Target Alias: KIAA0723; MAT3; MGC9105
Product Type Specific: MATR3 phosphosite-specific antibody
Antibody Code: PN883
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S188
Protein UniProt: P43243
Protein SigNET: MATR3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MATR3
Antibody Immunogen Sequence: FRRD(pS)FDD(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues S184 to D191. The effect of S188 phosphorylation is unclear. This is the major in vivo phosphorylation sites in MATR3 based on ≥388 mass spectrometry reports documented in PhosphositePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken.
Related Product 1: MATR3 S248+Y250 phosphorylation antibody (Cat. No.: AB-PN884)
Related Product 2: MATR3 S533 phosphorylation antibody (Cat. No.: AB-PN885)
Related Product 3: MATR3 S596+S598 phosphorylation antibody (Cat. No.: AB-PN886)
Related Product 4: MATR3 T150 phosphorylation antibody (Cat. No.: AB-PN887)
Scientific Background: MATR3 (Matrin 3) is an RNA binding and processing protein. It binds both DNA and RNA via two zinc-finger domains and two RNA Recognition Motifs (RRMs), often targeting pyrimidine-rich regions. MATR3 interacts with several proteins including DHX9 and HNRNPK, with its binding abilities heavily reliant on the RRM2 domain. It may play a role in transcription, or through two alternatively spliced isoforms in humans, may interact with other nuclear matrix proteins to form the internal fibrogranular network. It is primarily found in the nuclear matrix, where it binds to chromatin and maintains nuclear architecture. In association with the SFPQ-NONO heteromer, it may play a role in nuclear retention of defective RNAs. It functions in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, which serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. It binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. It can also bind to specific miRNA hairpins. Mutations in the MATR3 gene are associated with inherited amyotrophic lateral sclerosis (ALS) and distal myopathy. This description may include information annotated by UniProt and/or Google AI.