Product Name:
KIF5A-pS176
Product Number:
ab-pn882
Target Full Name: Kinesin heavy chain isoform 5A
Target Alias: Kinesin heavy chain 5A; Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain; NKHC
Product Type Specific: KIF5A phosphosite-specific antibody
Antibody Code: PN882
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S176
Protein UniProt: Q12840
Protein SigNET: KIF5A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human KIF5A
Antibody Immunogen Sequence: C(βA)RFVS(pS)PEE
Antibody Immunogen Description: Corresponds to amino acid residues R172 to E179. The effect of S176 phosphorylation is unclear. There are no reports of in vivo phosphorylation of this site in KIF5A based on mass spectrometry studies recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Fruit fly | Honey bee | Sea urchin.
Related Product 1: KIF5A S155 phosphorylation antibody (Cat. No.: AB-PN881)
Scientific Background: KIF5A (Kinesin Family Member 5) belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. It is a neuron-specific motor protein that acts as an ATP-dependent molecular motor to transport cellular cargo, such as organelles, proteins and RNA granules, along microtubules toward their plus ends (anterograde transport). It is required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). It consists of an N-terminal motor domain (ATPase activity/microtubule binding), a central coiled-coil stalk (dimerization), and a C-terminal tail (cargo binding). It acts as a dimeric motor, often working in conjunction with Kinesin Light Chains (KLCs). It is distributed throughout the CNS, but it is highly enriched in subsets of neurons. It can induce formation of neurite-like membrane protrusions in non-neuronal cells through formation of a ZFYVE27-KIF5A complex, which contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. KIF5A is also required for anterograde axonal transportation of MAPK8IP3/JIP3, which is essential for MAPK8IP3/JIP3 function in axon elongation. Mutations in the KIF5A gene disrupt intracellular transport and are linked to several, often severe, neurological conditions. Truncating mutations of KIF5A has been linked to amyotrophic lateral sclerosis (ALS) type 25. Mutations in the motor domain cause Hereditary Spastic Paraplegia (HSP) type 10. Mutations that affect the KIF5A's ability to properly transport materials can cause neonatal-onset intractable epilepsy/KIF5A-related disorder (KIF5A-RD).This description may include information annotated by UniProt and/or Google AI.