Product Name:
HNRNPA1-pY347
Product Number:
ab-pn880
Target Full Name: Heterogeneous nuclear ribonucleoprotein A1
Target Alias: FLI-2; HDP-1; Helix-destabilizing protein; HnRNP core protein A1; HnRNPA1; HnRNP-A1; HNRPA1; ROA1; Single-strand binding protein; TIS; Topoisomerase-inhibitor suppressed
Product Type Specific: HNRNPA1 phosphosite-specific antibody
Antibody Code: PN880
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y347
Protein UniProt: P09651
Protein SigNET: HNRNPA1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HNRNPA1
Antibody Immunogen Sequence: CGGGQ(pY)FAK
Antibody Immunogen Description: Corresponds to amino acid residues G343 to K350. The effect of Y347 phosphorylation is unclear. This is the major in vivo phosphorylation site in HNRNPA1 (≥622 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.7 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse.
Scientific Background: hnRNP A1 (Heterogeneous nuclear ribonucleoprotein A1) is a highly abundant, ubiquitously expressed RNA-binding protein. It is primarily localized in the nucleus, but constantly shuttles between the nucleus and cytoplasm to regulate gene expression, often acting as a component of ribonucleosomes. It is involved in alternative splicing, mRNA transport and stability, miRNA processing, and translation regulation. It also plays a role in telomere maintenance and maintaining a contractile vascular smooth muscle cell (VSMC) phenotype. It is composed of two N-terminal RNA-recognition motifs (RRMs) and a C-terminal low-complexity domain (LCD) that facilitates phase separation and stress granule formation. The protein is highly regulated by post-translational modifications (PTMs), which control its subcellular localization and activity in response to stress. Dysfunction of hnRNP A1 is associated with inclusion body myopathy, ALS, and frontotemporal dementia due to its role in cellular stress responses. Mutations in the gene (e.g., in the prion-like domain) are linked to ALS and other neurodegenerative diseases. It acts as a host factor for many viruses (e.g., influenza) to assist in replication, while providing an antiviral effect against others (e.g., HTLV-1). Overexpression of hnRNP A1 is common in various cancers, and may aid tumour progression. This description may include information annotated by UniProt and/or Google AI.