Product Name:
FUS-pS277
Product Number:
ab-pn876
Target Full Name: RNA-binding protein FUS
Target Alias: 75 kDa DNA-pairing protein; ALS6; FUS1; Fusion (involved in t (12; Fusion, derived from t(12;16) malignant liposarcoma; Heterogeneous nuclear ribonucleoprotein P2; Oncogene FUS; Oncogene TLS; POMp75; TLS; Translocated in liposarcoma; Translocated in liposarcoma protein
Product Type Specific: FUS phosphosite-specific antibody
Antibody Code: PN876
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S277
Protein UniProt: P35637
Protein SigNET: FUS
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human FUS
Antibody Immunogen Sequence: RHD(pS)EQD(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R274 to D280. S277 inhibits interaction with hnRNP P2. This is a minor site of phosphorylation with ≥10 mass spectrometry reports recorded in PhosphoSitePlus. FUS is known to be phosphorylated at this site in vitro by CK1d.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Platypus.
Related Product 1: FUS S340 phosphorylation antibody (Cat. No.: AB-PN877)
Related Product 2: FUS Y468 phosphorylation antibody (Cat. No.: AB-PN878)
Scientific Background: FUS (Fused in Sarcoma, a DNA-/RNA-binding protein that shares functional homology with TDP-43, which has also been associated with mutations linking to amyotrophic lateral sclerosis (ALS). Also known as hnRNP P2, it plays a role in various cellular processes such as transcription regulation, pre-RNA splicing, RNA transport, DNA repair and damage response. It binds to nascent pre-mRNAs and facilitates RNA polymerase II and U1 small nuclear ribonucleoprotein interaction to couple transcription and splicing. It binds also its own pre-mRNA and autoregulates its expression by non-sense-mediated decay. It shuffles between the nucleus and cytoplasm. It features an N-terminal prion-like domain (involved in aggregation), an RNA recognition motif (RRM), glycine-rich regions, and a C-terminal zinc finger domain and nuclear localization signal (NLS). Mutations in the FUS gene (often in the NLS) cause the protein to accumulate in the cytoplasm, leading to toxic, insoluble aggregates associated with neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD). FUS can form fusion proteins (e.g., FUS-CHOP) via chromosomal translocations, which are associated with various cancers like liposarcomas. This description may include information annotated by UniProt and/or Google AI.