Product Name:
DCTN1-pS541
Product Number:
ab-pn874
Target Full Name: Dynactin subunit 1
Target Alias: 150 kDa dynein-associated polypeptide; 150 kDa dynein-associated polypeptide;DP-150;DAP-150;p150-glued;p135; DAP-150; DCTN1; DP-150; DYNA; Dynactin 1 (p150, glued); DYNAN; P135; P150-glued
Product Type Specific: DCTN1 phosphosite-specific antibody
Antibody Code: PN874
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S541
Protein UniProt: Q14203
Protein SigNET: DCTN1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DCTN1
Antibody Immunogen Sequence: QEA(pS)VER(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q538 to R544. The effect of S541 phosphorylation is unclear. This is a minor site of in vivo phosphorylation with 4 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog.
Related Product 1: DCTN1 T1152+S1153 phosphorylation antibody (Cat. No.: AB-PN875)
Related Product 2: DCTN1 S105+T108 phosphorylation antibody (Cat. No.: AB-PN873)
Scientific Background: DCTN1 (Dynactin 1) is a motor protein that belongs to the dynactin 150 kDa subunit family. It functions in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. It binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. It is essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). It can also act as a brake to slow the dynein motor during motility along the microtubule. It regulates microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. It inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. It functions in metaphase spindle orientation, centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. It is also required for microtubule anchoring at the mother centriole. Defective signalling through DCTN1 has been linked to amylotrophic lateral sclerosis (ALS). This description may include information annotated by UniProt and/or Google AI.