Product Name:
ANXA11-pY482
Product Number:
ab-pn871
Target Full Name: Annexin A11
Target Alias: Annexin-11; ANX11; CAP50
Product Type Specific: ANXA11 phosphosite-specific antibody
Antibody Code: PN871
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y482
Protein UniProt: P50995
Protein SigNET: ANXA11
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ANXA11
Antibody Immunogen Sequence: CGKSL(pY)HDI
Antibody Immunogen Description: Corresponds to amino acid residues G478 to I485. The effect of Y482 phosphorylation in ANAX11 is unclear. It is the major site of in vivo phosphorylation by mass spectrometry (≥178 reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Mouse.
Related Product 1: ANXA1 Y207 phosphorylation antibody (Cat. No.: AB-PN503)
Related Product 2: ANXA2 Y238 phosphorylation antibody (Cat. No.: AB-PN504)
Scientific Background: ANXA11 (Annexin A11) belongs to the annexin family of calcium- and lipid-binding protein. It is a Ca2+-dependent, 56 kDa phospholipid-binding protein that mediates membrane trafficking and cellular homeostasis. It links membrane dynamics to cell division during mitosis, by translocating from the nucleus to spindle poles in metaphase, to the midzone in anaphase, and to the midbody in telophase. Disruption causes defects in cytokinesis, resulting in cell death. It also acts as a tether between lysosomes and stress granules. Pathological mutations impair this function, leading to the formation of protein aggregates in neurons. It binds specifically to calcyclin in a calcium-dependent manner. It features a conserved C-terminal core with 4 annexin repeats (Ca²⁺-binding) and a unique, long, intrinsically disordered N-terminal domain that acts as a scaffolding site for protein binding (e.g., S100A6/Calcyclin). Mutations in ANXA11, particularly in its N-terminal region, are linked to Amyotrophic Lateral Sclerosis (ALS). Besides ALS, it is also linked to sarcoidosis and is considered a marker of immune responses. This description may include information annotated by UniProt and/or Google AI.