Product Name:
TP73-pY99
Product Number:
ab-pn861
Target Full Name: Tumor protein p73
Target Alias: p53-like transcription factor; p53-related protein; P73; P73 alpha protein; p73-alpha; TP73; TRP73
Product Type Specific: p73 phosphosite-specific antibody
Antibody Code: PN861
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y99
Protein UniProt: O15350
Protein SigNET: TP73
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TP73
Antibody Immunogen Sequence: HSP(pY)AQP
Antibody Immunogen Description: Corresponds to amino acid residues H96 to P102. The effect of TP73 Y99 phosphorylation is unclear. This is a minor site of in vivo phosphorylation based on ≥1 mass spectrometry report recorded in PhosphoSitePlus. p73 is known to be phosphorylated at this site in vitro by Abl1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Scientific Background: TP73 (Tumour protein p73) is a member of the p53 family of transcription factors. It acts as a crucial tumour suppressor and regulator of cell stress responses, differentiation, and neurogenesis. It participates in the apoptotic response to DNA damage. It often exists in opposing TAp73 (pro-apoptotic) and delta-Np73 (anti-apoptotic) isoforms, influencing cancer progression and chemosensitivity. Similar to p53, p73 contains a transactivation domain, a DNA-binding domain, and an oligomerization domain. It binds to p53-responsive genes to induce cell cycle arrest and apoptosis. TAp73 contains the N-terminal transactivation domain, promoting apoptosis and acting as a tumour suppressor. Delat-Np73 lacks the N-terminus, acts as an oncogene by inhibiting TAp73 and p53. TP73 is also essential for nervous system development, particularly in the differentiation of multiciliated motile cells. Its activity is regulated by phosphorylation (e.g., by ABL1, PLK1) and degradation through the proteasome. It is expressed in striatal neurons of patients with Huntington disease, and found in rain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. It is highly expressed in fetal tissue. p73 is rarely mutated in human cancers, but its expression is dysregulated, with high Delta-Np73/TAp73 ratios often indicating poor prognosis. This description may include information annotated by UniProt and/or Google AI.