Product Name:
Tau-pT529+pT534 (pT212+pT217)
Product Number:
ab-pn857
Target Full Name: Microtubule-associated protein tau
Target Alias: DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MAPTL; MGC138549; Microtubule-associated protein tau, isoform 4; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; PPND; TAU; Tau
Product Type Specific: Tau phosphosite-specific antibody
Antibody Code: PN857
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T529+T534
Protein UniProt: P10636
Protein SigNET: Tau
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Tau
Antibody Immunogen Sequence: C(βA)RSR(pT)PSLP(pT)PP
Antibody Immunogen Description: Corresponds to amino acid residues R526 to P531. The effects of Tau T529 and T534 phosphorylation are unclear. These are major in vivo phosphorylation sites based on ≥22, and 46 mass spectrometry reports, respectively, in PhosphoSitePlus. Tau is known to be phosphorylated in vitro at T529 by BRSK1, BRSK2, CDK5, GSK3b, and at T534 by CDK5, and GSK3b.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken.
Related Product 1: Tau S515+S516+S519 phosphorylation antibody (Cat. No.: AB-PN856)
Related Product 2: Tau T529+T534 phosphorylation antibody (Cat. No.: AB-PN857)
Related Product 3: Tau S713 phosphorylation antibody (Cat. No.: AB-PN858)
Scientific Background: Tau protein is a microtubule-associated protein, primarily found in neurons, that is encoded by the MAPT gene. It stabilizes the cytoskeletal structure, aiding in nutrient transport and cell shape maintenance. It acts in the central nervous system to support axonal structure. It promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules, while the N-terminus binds neural plasma membrane components, such that tau may function as a linker protein between both. Axonal polarity is predetermined by Tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Normally, it is a highly soluble, natively unfolded protein, and humans exists as six major isoforms with either three (3R) or four (4R) microtubule-binding repeats. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Isoform PNS-tau is expressed in neurons in the peripheral nervous system while the others isoforms are expressed in the central nervous system. In certain diseases, Aau becomes abnormally modified (e.g., hyperphosphorylated). This causes it to detach from microtubules and aggregate into insoluble neurofibrillary tangles. This can lead to neurodegenerative diseases (tauopathies) like Alzheimer’s disease, frontotemporal dementia, and other neurodegenerative conditions. These tangles are associated with synaptic dysfunction, neuronal death, and cognitive decline. This description may include information annotated by UniProt and/or Google AI.